The Effects Of 2-deoxy-D-ribose On The Expression Of GLUT1/4 In Breast Cancer Cells

ObjectiveTo investigate the effects of 2-deoxy-D-ribose on breast cancer cell lines,MDA-MB-231 and MCF-7,and to explore the possible mechanism of glucose uptake and glucose transportation.MethodsBreast cancer cell,MDA-MB-231 and MCF-7,were cultured in different concentrations(0,1,100,103,104,105?M)for 2dDr for different time(12h,24h).The cell viability of MDA-MB-231 and MCF-7 cells was detected by cell counting kit(CCK-8).The cell migration and invasion abilities were detected by Transwell method.The glucose-uptake assay was performed by 2-NBDG method.The expression of CREB,p-Akt were detected by Western blot.The total cellular protein and membrane protein were extracted respectively.The expression of GLUT1/4 in cell cytoplasm and cell membrane was detected and detected by immunoblotting.The distribution of GLUT 1/4 was detected by immunofluorescence.The cells were treated with 0,103?M 2dDr and inhibitor SB203580 or PD98059,then were detected the expression of GLUT1 and GLUT4 by Western blotting.Results2dDr treatment increased the cell viability of breast cancer cell line MDA-MB-231,and had no significant effect on the cell viability of MCF-7 cells.In vitro experiments,2dDr had no significant effect on the migration and invasion of MDA-MB-231 and MCF-7 cells.After treated with 2dDr for 12h,the glucose uptake capacity of MDA-MB-231 decreased slightly,but there was no significant difference,and the glucose uptake capacity of MCF-7 cells did not change significantly.The expression of GLUT1 in MDA-MB-231 was significantly improved after treated with high concentration of 2dDr(104,105?M)for 24h,the expression of GLUT4 was obvious in the nucleus,the expression of CREB was increased,and the phosphorylated Akt were elevated.In MCF-7,the expression of GLUT1 and GLUT4 were increased with the growth of concentration of 2dDr.With the increase of concentration of 2dDr,the increase of expression of CREB and the phosphorylated Akt increased with the growth of concentrationof 2dDr in MCF-7.The membrane protein content of GLUT1 and GLUT4 did not change significantly in both cell lines,and the cytoplasm content increased with the increase of 2dDr concentration in MDA-MB-231 but not in MCF-7.In MDA-MB-231,SB203580 treatment blocked 2dDr(103?M)-induced GLUT1/4 upregulation;PD98059 treatment also significantly inhibited 2dDr(103?M)-induced increase in GLUT1/4.In MCF-7,SB203580-treated inhibition of 2dDr(103?M)-induced GLUT1/4 was not significant;PD98059 treatment did not show significant inhibition of 2dDr(103?M)-induced GLUT1/4 upregulation.ConclutionThe increase of 2dDr concentration could improve the expression of GLUT 1 and GLUT4 in MDA-MB-231 and MCF-7,but could not significantly affect the glucose uptake,for the reason that 2dDr could not improve the localization of GLUT1 and GLUT4 cells in the cell membrane.