Biomimetic Preparation And Cytological Research Of Organic-inorganic Multi-hybrid Magnetic Bone Repair Materials

Compared with other composites,organic-inorganic bioactive hybrids were more widely researched,which were mainly applied in the repairing and rebuilding of human defect issue.Currently,the study of the magnetic composite materials shows that the magnetic field with the magnetic composite scaffolds can significantly affect the cell growth state of the organism.In this study,in order to prepare hybrid nanostructure composite,combining with the superior characteristics of chitosan(CS)and collagen(Col)as the organic matrix,the soluble calcium salt and soluble phosphate as the precursor of inorganic phase nano hydroxyapatite(nHAP),and the soluble salt and soluble ferrous salt as precursor to inorganic phase superparamagnetic nano Fe3O4 particles were used to prepare magnetic composite scaffold by organic-inorganic hybrid technology according to freezing drying technique and in situ hybridization.This experiment builded CS/Col/nHAP and CS/Col/Fe3O4/nHAP in situ composite scaffolds,which the ratio of Fe2+and Fe3+ is different,recorded as R1 and R2,respectively.The physical and chemical properties of biphase hybrid micro and nano composite materials with micro and nano structure was analyzed by SEM,FTIR,XRD,physical performance tester(PPMS)and degradation performance,et al.SD neonatal rat calvarial osteoblasts were isolated and cultured by two enzyme digestion-continuous tissue block method,and cultured with the composite scaffolds to build the evaluation system to investigate its biocompatibility.Biocompatibility was tested by giemsa's staining,CCK-8 and AO/EB methods.SEM showed that the composite scaffolds had highly connected porous structure.And the pore size is about 100?150 ?m.As shown in XRD,FTIR and XPS,there are two kinds of diffraction peaks which are consistent with the characteristic peaks of nHAP crystal and nano Fe3O4 particles,respectively.And it can also be concluded from the XRD diagram that the crystallinity of nHAP is lower,which is similar to nature bone.Combined with SEM,XRD and FTIR analysis,it can be concluded that nHAP and nano Fe3O4 particles have been synthesized in situ on the composite scaffolds.And the porosity of these composite scaffolds were 90.5%,98%,and 92.8%,respectively.The results of in vitro degradation and mineralization showed that the composite scaffolds had good degradation and mineralization activity.And the magnetic properties showed that the saturation magnetization was 0.025 emu/g,and have good magnetic response.The results of in vitro culture system showed that the composite scaffolds could promote cell division,proliferation,growth and metabolic activity.Based on the above experimental results,biphasic hybrid magnetic bone repair material with micro and nano structure has benign biocompatibility.And it is promising for bone repair application in bone tissue engineering.