Application Of SERS Nanoprobe Technology In Label-Free Lung Cancer Detection

With the continuous air pollution,smoking rates rise and other factors,lung cancer gradually developed into the world's morbidity and mortality of the highest one of the malignant tumors.Because of the lack of early onset of the disease specific symptoms and signs,and routine application of sputum cytology,tumor markers and chest imaging studies are often limited to the low positive rate,cumbersome detection methods,radiation damage or false positive interference Shortcomings,patients oftenmiss the best treatment time,resulting in poor prognosis of patients,how to find a safe and convenient,non-radiation non-destructive,highly sensitive and specific detection methods have become the focus of research in recent years.In this study,the most sensitive diagnostic marker cytokeratin 19 fragment(CYFRA21-1)for non-small cell lung cancer was selected as the research object.We combined surface enhanced Raman scattering(SERS)with enzyme linked immunosorbent assay(ELISA)to establish a new method for detection of lung cancer tumor markers.In the first step of the study,we assembled nanosized gold with 5,5'-dithiobis(DSNB)and CYFRA21-1 antibody into SERS nano-probes,followed by detection of CYFRA21-1 antigen on a substrate made using the method of nano-gold-silver staining,In this experiment,we found that the Raman band intensity of DSNB was exponentially linear with the concentration of CYFRA21-1 antigen.The method has the advantages of simple steps and high sensitivity,and can realize the qualitative and quantitative detection of the CYFRA21-1antigen.