Identification Of Hepatitis B Virus X Gene Integration In Hepatocellular Carcinoma Tissues Of Guangxi

Objective : To investigate integration of hepatitis B virus(HBV)X gene in chromosome of primary hepatocellular carcinoma(HCC),common integrated and to identification the function in the development of HCC.Methods:Alu repeat Sequence-Polymerase Chain Reaction was used to identify the HBV integration in 30 pairs of HBV-related HCC tissues and adjacent non-tumorous liver tissues.The Alu-PCR products were purified and subjected to direct sequencing.NCBI(National Center for Biotechnology Information)BLAST and Map Viewer research were used for identification of HBV location on human genomes.Result:Among 30 HCC patients studied,in 17(56.67%)HBV-related HCC tissues with integration,forward insertions of HBV X DNA into the host chromosomal were found in 2 samples,and reverse insertions were found in 12 samples while both forward and reverse in insertions were found in 3 samples.In 26(86.67%)adjacent non-tumorous liver tissues with integration,forward insertions of HBV X DNA into the host chromosomal were found in 7 samples,and reverse insertions were found in 7 samples while both forward and reverse in insertions were found in 12 samples.The difference of HBV-related HCC tissues and adjacent non-tumorous liver tissues of HBV integration was statistically significant(P<0.05).Among 30 HCC patients studied,HBV-related HCC tissues localization of 11 integration sites had 8 corresponding genes,P73?TGFRBR2?h TERT?HRAS?HNF1A ? P53 ? TTR ? BSG ? MLL4;adjacent non-tumorous liver tissues localization of 27 integration sites had 17 corresponding genes,P73?IL10?IRS1?MSH2?hTERT?CDKN1A? KDM4C?IL2RA?HRAS?HNF1A?EDNRB?MAPK3?IL4R?CEPT?TTR?BSG?EP300.The HBV DNA integration was nothing to do with the sex,age,HBeAg,HBV DNA level,ALT level and AST level(P>0.05).Conclusion:HBV X gene integration is not distributed evenly throughout the host chromosome.Three genes,P73,hTERT,HARS are common integration for HCC in GuangXi.Common integration genes closely relate to the occurrence of HCC.