Study On The Action Process Of Cistanche Deserticola Total Glycosides Based On Liver Metabolism

This subject is based on the previous work,has confirmed that Cistanche deserticola has the estrogenic effect,among them,Cistanche deserticola total glycosides is the primary active part,and Cistanche deserticola total glycosides obtained by optimized,content of Acteoside is 62.5%.In this subject,liver metabolism is the study model because of that is the main way of body's metabolism,establish a metabolic map of Intrinsic component—into Blood—Liver—Bile,infer the regularity of qualitative change of Cistanche deserticola total glycosidesmetabolism by liver,elucidate the internal metabolic process of the Cistanche deserticola total glycosides,reveal further the efficacy material basis and action process of Cistanche deserticola total glycosides in the body.(1)Using the UPLC/Q-TOF-MS technology to analyze the blood components of Cistanche deserticola total glycosides,in the previous study,43 original components were demarcated and 78 chemical components were identified totally,including 5 archetypay components are as follows ?-sitosterol?Diosgenin?Syringalide?crenatoside?Kankanoside E,73 kinds of metabolic components are 8-epiloganic acid sulfuric acid bond?acetylation Syringaresinol-O-?-D-glucopyranoside?methoxy 8-epiloganic acid?methylation Salidroside?go to the hydroxylation Bartsioside?Kankanoside L sulfuric acid bond?methoxy 6-deoxy Catalpol?methylation Geniposide?methoxy Syringalide?Dulcitol sulfuric acid bond?acetylation Syringalide?6-deoxy Catalpol sulfuric acid bond?Kankanoside A sulfuric acid bond?methoxy Geniposidic acid?acetylation Ononin?hydroxylation Cistanoside F?methylation Tubuloside B?Cistanoside G glucuronic acid conjugate and so on.Their metabolic pathways are mainly methoxy?sulfate?acetylation?methylation?hydroxylation?go to the methylation?hydroxylation?glucose aldehyde acidizing.(2)Liver metabolism is the main mode of metabolism in the body,in this subject,liver metabolism is used as the main model to study the metabolic map of liver homogenate after liver metabolism,78 chemical components were identified in total,including 7 prototypes are as follows 8-epiloganic acid?Decaffeoyl acteoside?6-deoxy Catalpol?Kankanoside L?Sinapaldehyde glucoside?dehydrodiconiferyl alcohol-4-O-?-D-glucoside?Echinacoside,71 kinds of metabolites are acetylation ?-sitosterol?8-epiloganic acid sulfuric acid bond?go to the methylation Kankanoside E?go to the hydroxylation Salidroside?methylation Salidroside?methoxy Kankanoside A?acetylationSyringalide?Kankanoside A sulfuric acid bond?methylation ?-sitosterol?hydroxylation Kankanoside M?methoxy Cistanoside G?acetylation Cistanoside G?hydroxylation Cistanoside G?Syringalide sulfuric acid bond?hydroxylation Sinapaldehyde glucoside?Sinapaldehyde glucoside glucuronic acid conjugate and so on.Their metabolic pathways are mainly sulfate?acetylation?methylation?hydroxylation?go to the methylation?go to the hydroxylation?glucose aldehyde acidizing.(3)Combined with LC/MS technology,compared with the previous work foundation of the research group,we identified 48 chemical components in the bile metabolism map after liver metabolism,of which 2 prototypes are Cistanoside G?dehydrodiconiferyl alcohol-4-O-?-D-glucoside,46 kinds of metabolites are Dulcitol sulfuric acid bond?acetylation Cistanoside G?hydroxylation 8-epiloganic acid?Cistanoside G sulfuric acid bond?go to the methylation Cistanoside E?hydroxylation crenatoside?Dulcitol glucuronic acid conjugate?methylation Syringgalide?go to the methylation Daucosterol and so on.Their metabolic pathways are mainly sulfate?acetylation?methylation?hydroxylation?go to the methylation?go to the hydroxylation?glucose aldehyde acidizing.(4)In this part of the experiment after intragastric administration of Cistanche deserticola,collect of samples of serum,liver homogenate and bile to annalyze,using the UPLC/Q—TOFMS technology,comparing the components in the serum,liver homogenate,and bile samples,we know that there are 9 components as follows Cistanoside F sulfuric acid bond?hydroxylation ?-sitosterol?acetylation Cistanoside G?methoxy Tubuloside B?Cistanoside G glucuronic acid conjugate?acetylation Mussaenosidic acid?methoxy 2'-acetylacteoside?methylation Coniferin?methoxy Sinapaldehyde glucoside.Their metabolic pathways are mainly sulfate?glucose aldehyde acidizing?hydroxylation?acetylation?methoxy.