Clinical And Basic Experimental Study Of Wind In The Treatment Of Nasal Virus Upper Respiratory Tract Infection

Objective:In this study the wind cough in vitriol anti virus and anti inflammation of the nose,wind cough for anti rhino-virus,anti-inflammatory and recrudescence effects from the experimental data;in clinical trials,by observing the wind cough for the treatment of rhino-virus infection leads to the improvement of clinical symptom,patients with upper respiratory tract infection symptoms of traditional Chinese medicine integral,the effective rate of the treatment,the wind cough objective evaluation for clinical treatment of nasal viral upper respiratory tract infection is safe and effective,and the results were analyzed and the technology,and ultimately provide the basis for Chinese medicine treatment of rhino-virus infection of upper respiratory tract.Methods:1.Experimental study on the anti retrovirus in vitriolWith the wind cough as the treatment group,Leigh B have Lin as a positive control group,in human sadomasochism cells for virus host cell samples inhibited rhino-virus induced human sadomasochism cell lesion degree.Daily use of inverted microscope examination and record cell lesions,observe the maximum non-toxic concentration of samples by CPE method(TDO);calculate half toxic wind cough by Reed-Muench method(TD50);calculate the wind cough half of nasal virus inhibitory concentration by Reed-Muench method(IC50);SI drug selection index calculation the(SI=TD50/IC50).2.Experimental study on anti inflammationBy peritoneum injection of acetic acid induced mice capillary permeability increase,at the same time to mice by intravenous injection of Evans blue and Evans blue exudation from the blood vessels to observe abdominal volume,to study the differences of different doses of wind cough inhibit the acute inflammation early increased vascular permeability;by smearing ethylene to cause one auricle of the mouse ear swelling model,determination of two ears the weight difference is the ear swelling degree to the reagent of different doses of early inflammation inhibition.3.clinical study of wind cough sideTake clinical trial design randomized,positive control,subjects in our hospital from August 2015 to August 2016 respiratory outpatient and emergency department patients diagnosed in 120 cases of rhino-virus infection of the upper respiratory tract of patients,were randomly divided into experimental group(wind cough group)and control group(Ribaldry Effervescent Granules group),each group of patients were 60 cases.The treatment group was treated with wind cough with disease(composed of Ephesus 10g,10g,10g,almond Schizophrenia aster 10g,peril la 10g,10g,10g,Interstellar Plectrum 10g,lOg licorice,Terminable 10g),an agent,water decoration 500ml,sooner or later.control group received daily oral 150mg Ribaldry Effervescent Granules 3 times.Results:Wind cough in vitriol anti rhino-virus found quite at low concentrations downwind cough particles and Leigh B have Lin antiviral effect,its IC50 was 0.0061 mg/ml and 0.0059 mg/ml,but in the historicity test,the toxicity of wind cough particles on the cell below Leigh B have Lin,drug wind cough particle selection index SI(967.41)to calculate Leigh B have Lin(564.97)was significantly higher than that of.Therefore,the wind cough particles in vitriol with low toxicity and high efficiency of anti rhino-virus effect.Found anti swelling experiment,wind cough in inhibition of capillary permeability in mice induced by acetic acid in hyperthyroidism,and wind cough reduce ear swelling of mice induced by two experimental results showed that the ethylene,the wind cough granules in high dose(23 g crude drug/kg)and low dose(5.75g crude drug/kg)significantly reduced Joe Meningitis mice capillary permeability,effectively reduce the exudation of Evans blue from the blood vessels;high dose(23g crude drug/kg)can significantly reduce the mice ear swelling,inhibit the ear swelling rate of 38.4%.Therefore,the reagent has an obvious inhibitory effect on early acute inflammation.The clinical study found that patients with the wind cough Decoration in the treatment of rhino-virus infection of the upper respiratory tract before and after treatment clinical symptoms showed that the experimental group(wind cough group)the clinical curative effect of patients with the total effective rate was 88.3%;the control group(Leigh Behave Lin group)the clinical curative effect of patients with the total efficiency of 73.39%;the treatment group the total effective rate and the total efficiency of the control group was statistically significant(P<0.05).The wind cough clinical curative effect is obviously superior to Ribaldry Effervescent Granules treating wind evil rhino-virus infection of the upper respiratory tract.In the improvement of TCM syndrome integral,the TCM syndrome score of wind cough group was significantly improved than that of the control group,and the wind cough group could reduce the body temperature of the experimental group more quickly and effectively.Conclusion:The wind cough of human sadomasochism cell lesions caused by inhibition of rhino-virus,and treatment of rhino-virus infection of upper respiratory tract disease,the symptoms and the total efficiency was significantly better than the Leigh B have Lin group,the two comparison has statistical significance(P<0.05);in the two anti swelling experiment,show the wind cough granule high dose(23 g crude drug/kg)can significantly reduce the capillary permeability of mice and significantly reduce the mice ear swelling,visible,the reagent has an obvious inhibitory effect on early acute inflammation.The clinical research also shows that the wind cough groups in improving TCM syndrome in patients with clinical symptoms,symptoms,fever are significantly better than the Ribaldry Granules group.