| Objectives1.To study on the changes of serum resistin in patients with type 2 diabetes mellitus?T2DM?;2.To study the effect of serum resistin on blood glucose,blood lipid metabolism and insulin effect.3.To investigate the risk genes of resistin rs2161490 and rs1423096 in patients with type 2 diabetes in Guangdong.4.To study on the single nucleotide polymorphisms of the two loci of resistin gene rs2161490 and rs1423096 were studied in the distribution of type 2 diabetic patients in the Guangdong region and the normal control group.research method1.Experimental groupingFrom January 2015 to November,to come from the Second Affiliated Hospital of Guangzhou Medical University,and was first diagnosed with type 2 diabetes patients selected qualified 178 cases,including 78 cases of male patients,100 female patients,mean age?61±11?years old,case patients required:we must first meet the diagnostic criteria of type 2 diabetes,the development of WS397-2012 screening and diagnosis criteria for diabetes include:1.the symptoms of diabetes and random blood glucose greater than or equal to 11.1mmol/L;2.fasting blood glucose than 7.0mmol/L;3.OGTT,2 hours blood glucose over 11.1mmol/L.If the patient is a special type of diabetes or type 1 diabetes,or have severe organ?liver,heart,kidney,etc.?failure,have diseases caused by metabolic problems can not be admitted to the case group.192 healthy people who had physical examination at the same time period were selected as the control group,including 78 males and 114 females,with an average age of 66±7 years.The control group required that both fasting blood glucose and 2hours postprandial blood glucose were in the normal range,and no diseases caused by metabolic problems.2.Experimental testing project?1?The case group and the control group,fasting blood glucose,total cholesterol,serum low-density lipoprotein cholesterol,glycerin three fat and high density lipoprotein cholesterol levels were measured in Roche C8000 automatic biochemical analyzer,Quality control product is Roche supporting quality control product;in the United States ELX800 automatic enzyme mark analyzer on serum resistin concentration.?2?Detection by time-of-flight mass spectrometry:In the cases of type 2 diabetes group and healthy control group of resistin gene loci rs2161490 and rs1423096genotyping,analysis of the distribution of each genotype,and analysis whether whether the incidence of type 2 diabetes is associated SNPs and SNPs exist.3.Data analysisSPSS13.0 analysis software is used to process and analyze the experimental data.Hardy Weinberg's law examination group meets the genotype genetic equilibrium,The frequency of occurrence of each genotype in case group and control group was statistically analyzed byc?17?test.The serum lipid levels and resistin levels in case group and control group were measured by t test,The relative risk of various genotypes with type 2 diabetes:using the 95%confidence interval and ratio ratio to indicate the relative risk of type 2 diabetes.Serum resistin level and serum lipid level index were expressed by`X±sThe serum lipid levels of each genotype were analyzed by independent t test for each other comparative analysis.The difference between data statistics and P<0.05 was statistically significant.Experimental results1.Serum resistin levelThe healthy control group the average concentration of serum resistin level was 6.8133±0.7819 ug/L,the average concentration of serum resistin levels in type 2 diabetes group is 30.450±3.2446 ug/L,than the average level of the healthy control group increased significantly,the difference was statistically significant?t=6.736,P<0.05?.2.The frequency of resistin genotypes rs2161490and rs1423096The distribution of genotype frequencies of two loci was detected by the software of Hardy-Weinberg's genetic equilibrium law.The results showed that the samples from type 2 diabetes group and healthy control group reached the genetic balance,that is,group representation.There was no significant difference in the distribution frequency of resistin gene locus rs2161490 and rs1423096 genotype in type 2diabetes group and healthy control group?P>0.05?.3.Analysis of the relative risk of various genotypes and type 2 diabetesRegression analysis showed that there was no significant difference in relative risk between type2 diabetes and resistin rs2161490 and rs1423096 genotype?P>0.05?.The relative risk of type 2 diabetes was not statistically significant.4.The cases of type 2 diabetes group triglyceride was higher than healthy control group,and the high density lipoprotein is lower than the normal group,the difference was statistical significance?P<0.05?;serum lipid levels in type 2 diabetes group resistin loci rs2161490 and rs1423096 genotypes and the corresponding analysis of four blood lipid level of patients was rs2161490 and rs1423096 of each gene locus of resistin the corresponding analysis of single factor variance test to compare the difference of serum lipid levels,corresponding to each genotype were not statistically significant?P>0.05?.Conclusion1.The level of serum resistin in type 2 diabetes group was significantly increased?30.450±3.2446 ug/L?,which was significantly different from that in healthy control group?6.8133±0.7819 ug/L??P<0.05?,It is suggested that high level of serum resistin may be associated with the development and development of type 2 diabetes.2.The case group triglyceride and high density lipoprotein were compared with the control group,there was statistical significance?P<0.05?;four cases of resistin rs2161490 blood lipids and rs1423096 genotypes corresponding to the 22independent samples t tests were used to compare the results,four lipid genotypes corresponding to the differences were not statistically significant?P>0.05?,so it is concluded that the patients of resistin rs2161490 and rs1423096 loci for each genotype had no effect on the expression level of blood lipid.3.The T2DM case control group genotype of resistin rs2161490 and rs1423096 gene polymorphism in the group and the health of the frequency,risk degree,in the case of T2DM group and the healthy control group had no statistical significance,so as to deduce resistin rs2161490 and rs1423096 genotype is not the risk gene of type 2diabetes in Guangdong area. |
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