The Antifertility Effectiveness Of A Novel Copper-containing Composite And Its Influence On The Endometrial Environment Of Rabbits

Part?The antifertility effectiveness of a novel copper-containing composite and its capacity to restore fertility of rabbits after removalObjective To investigate the antifertility effectiveness and reversibility of a new and potential intrauterine device component material that contain cupric chloride/silicon dioxide/poly?vinyl alcohol??CuCl₂/SiO₂/PVA?in rabbits.Methods Forty-eight mature female white rabbits were randomly divided into four groups:bulk copper group?Cu group,n=12?,CuCl₂/SiO₂/PVA group?n=12,copper ion was released from IUD at a rate of 5–10 mcg/220 mm² per day?,SiO₂/PVA group?n=12?and shame operate group?SO,n=12?.All the materials were inserted into the uterus of rabbits after acclimatization of 1 week.Six rabbits in each group were mated with male rabbits of proven fertility from 30 days after insertion,and the antifertility rates were observed at Day20 of pregnancy.Meanwhile,six rabbits in each group were selected for removal of corresponding materials,and then mated again to determine their fertility restoration.Results Both the Cu group and the CuCl₂/SiO₂/PVA group had 100%antifertility rates,and both of these rates were significantly higher than that in the other groups?p<0.05?.All the rabbits in CuCl₂/SiO₂/PVA group were pregnant after the materials removed and there were no differences between the Cu group and the CuCl₂/SiO₂/PVA group?p>0.05?.Conclusion The novel CuCl₂/SiO₂/PVA composite exhibits effective and reversible antifertility as reliable as traditional material.Part ? The influence of a novel copper-containing composite on the endometrial environment of rabbitsObjective To observe the morphological changes and the expression levels of factors associated with bleeding and pain in the endometrium of rabbits,after being inserted with a novel copper-containing composite.Methods N ine mature female white rabbits were randomly divided into three groups: bulk copper group?C u group,n=3?,Cu C l2/Si O2/PVA group?n=3,copper ion was released from IUD at a rate of 5–10 mcg/220 mm2 per day?and shame operate group?SO,n=3?.All the materials were inserted into the uterus of rabbits after acclimatization of 1 week.The Histological analysis of major organ tissues and endometrium of each group was conducted at day 60 after insertion.The expression of substance P?SP?and vascular endothelial growth factor?VEGF?in the endometrium was examined by immunohistochemistry and western-blot at day 60 after insertion.The concentrations of prostaglandin E2?PGE2?and tissue plasminogen activator?t PA?in the endometrium were measured by ELISA at day 60 after insertion.Results The morphology of major organ tissues in each group was nearly normal.The damage to the endometrial morphology caused b y Cu C l2/Si O2/PVA composite was much less than that caused by copper.The significant differences for SP and VEGF expression levels between the Cu C l2/Si O2/PVA group and the sham operate group?P<0.05?were identified and the changes of C u C l2/Si O2/PVA group on these parameters were less remarkable than those of the C u group?P<0.05?.Compared with those in the control group,the concentrations of PGE2 and t PA in Cu C l2/Si O2/PVA group were significantly higher?P<0.05?,and the changes of Cu C l2/Si O2/PVA group on these parameters were less remarkable than those of the Cu group?P<0.05?.Conclusion Compared with the conventional copper material,this novel Cu Cl2/Si O2/PVA composite has fewer adverse effects.