Study On The Preparation Process And Content Determination Of Shenmai Extract

Objective:Studies have shown that ginsenosides and polysaccharides are rich in pharmacological activities.Ginsenosides Rg₃ and Rh₂ have significant immunity b-oosting effects,but they only exist in red ginseng and are extremely low in content.T-his article aims to obtain the Shenmai extract,which has rich saponins,polysaccharid-es and high content ginsenosides Rg₃ and Rh₂,and the extract content of research.Methods:The extraction method was determined by comparing the total ginsenosides and total saccharides content of different extraction methods of red ginseng and Ophiopogon japonicum.The effects of extraction methods of red ginseng and Ophiopogon japonicum.The effects of extracting solvents,extraction time and extraction times on total ginsenosides and total saccharides yield were studied by ortho gonal test method,and the best extraction process was optimized.The ginsenosides and saccharides were separated by the macroporous resin method,and the related parameters were used to purify the saponin.The solvent amount and extraction time parameters in the extraction process of the best extraction process liquid ginsenosides by the macroporous resin method as the substrate for the conversion process.Examine the parameters of transformation process in the biotransformation method and the acid transformation method and comparing the effects of the two optimal conversion processes,then determine the ways and methods of the conversion process.The content of extracts was determined by spectrophotometric method,sodium periodate titration and high performance liquid chromatography.Results:1.Red ginseng and Ophiopogon japonicus were extracted by combine-d method,the results showed that the best extracting process was 5 times purified wa-ter for 3 times,1.5 h each time.The content of total ginsenosides is about 2.90%,the content of total polysaccharides is about 52%,and the solids content is about 53%.2.Using AB-8 macroporous resin separation method to examine the purification process of red ginseng and Ophiopogon japonicus extracted liquid.The maximum sample volume was 0.7 BV(about 0.5 g·mL^-1of the sample solution).The sugar volume was 3 BV that washed with water,the final 95%ethanol eluted saponin volume was 4.5 BV,and the total ginsenosides content after purification was approximately 2.49%.3.The optimum extraction process of ginseng stems and leaves was extracted with 10 times,8 times,and 8 times purified water for 3 times,each time for 1 hour,and which includes 0.34%of Rb₁ extract was finally obtained.The purification process also used AB-8 macroporous resin method.The maximum sample volume is2 BV(on the sample solution is about 0.2 g·mL^-1),2 BV of purified water is used to remove sugar and other substances,3 BV is washed with 20%ethanol solution of 0.5mol·L^-1 NaOH,and 3.5 BV purified water adjusts the pH to neutral,6 BV of 70%ethanol eluted the ginsenosides,finally obtaining a purified liquid containing 0.29%of ginsenoside Rb₁.4.The optimum transform process of ginsenosides is acetic acid transformation.When the concentration of acetic acid is 15%,the reaction temperature is between90°C and 100?,and the reaction time is 1.5h,about 0.99%to 1.10%of ginsenosides Rg₃ and Rh₂ can be obtained.5.The contents of the three extracts were determined.The results showed that it contained 25.07%total ginsenosides,67.72%total polysaccharides and 11.79%ginsenosides Rg₃ and Rh₂.Conclusion:1.Through the comparison of extraction method and orthogonal test method,total ginsenosides and total polysaccharides were taken as indexes to comprehensively examine and establish the optimal extrac tion process for red ginseng and Ophiopogon japonicus.2.Using AB-8 macroporous resin method,the purification process of red ginseng and Ophiopogon japonicus was established,and the total ginsenosides extract and the total polysaccharides extract were obtained.3.The optimum extraction process and the optimal purification process of ginseng stems and leaves were established,and ginsenoside Rb₁ was used as the inspection index.4.A high performance liquid chromatographic method for the simultaneous detection of rare saponins 20?S?-Rg₃,20?R?-Rg₃,20?S?-Rh₂,and 20?R?-Rh₂ was established and systematically examined in the transformation process.The parameters determine the acetic acid conversion process with the best transform effect and suitable for production.5.The contents of the obtained extracts were studied to provide a reference basis and scientific basis for the content control of the extracts in the project.