Preparation,Fractal Structure Characterization And Hypoglycemic Effect Of Selenium Momordica Charantia Polysaccharide

In previous studies in the laboratory,it was found that Momordica charantia polysaccharides have the effects of lowering blood sugar and relieving symptoms in diabetic rats and mice,and have correlations with their antioxidative activities.However,the solubility of the purified Momordica charantia polysaccharide becomes poor,and the poor solubility limits the exertion of the biological activity of the Momordica charantia polysaccharide.Selenium is an essential trace element in the body.In patients with diabetes,selenium is a relatively scarce trace element.However,the presence of selenium in the natural world is dominated by inorganic selenium.Since inorganic selenium carries some harmful groups to the organism,it is obviously incorrect to directly supplement inorganic selenium.Therefore,the selenium element is combined with the polysaccharide of Momordica charantia which has hypoglycemic activity,low toxicity and high oxidation resistance,and is the focus of the research of this subject.In this study,selenium is used as a modifier,a polysaccharide of Momordica charantia,using the principle of sodium selenite to reduce the selenium under the action of ascorbic acid.The binding state of selenium and Momordica charantia polysaccharides was detected by infrared spectroscopy and Raman spectroscopy.The surface structure of polysaccharideselenium was observed by scanning electron microscopy and transmission electron microscopy.Diabetic mouse model was used to study the hypoglycemic effect of polysaccharides-selenium in Momordica charantia.The following are the main contents of this topic:1.Sodium selenite reduces the selenium element under the reducing action of the reducing agent ascorbic acid,and the reduced elemental selenium is bound to the polysaccharide in the presence of the polysaccharide,namely,the bitter melon polysaccharide-selenium.When the reaction temperature is 40°C,the reaction time is 3 hours,and the polysaccharide concentration of Momordica charantia is 0.8 mg/ml,the molar ratio of sodium selenite to ascorbic acid is 1:3,stable selenium polysaccharide can be prepared.2.Infrared detection and Raman spectra of polysaccharides from selenium polysaccharide and polysaccharides from Momordica charantia showed that the elemental selenium reduced from sodium selenite was successfully bound to the surface of selenium polysaccharides,causing displacement of some carboxyl absorption peaks,indicating that selenium is a combination of the carboxyl group on the surface of the Momordica charantia polysaccharide.3.Scanning electron microscopy and atomic force microscopy of Momordica charantia polysaccharides and Momordica charantia polysaccharide-selenium: There are more sharp protrusions on the surface of the polysaccharides of the Momordica charantia Combined with the scanning electron microscopy image of the Momordica charantia polysaccharides,the surface of the polysaccharides of the Momordica charantia is in a state of sheet-like stacking.The corners of the shape are sharp.From the Momordica charantia polysaccharide-selenium atomic force microscopy photograph,it can be seen that the surface is smoother than the surface of the Momordica charantia polysaccharide,which is similar to covering a layer of milky substance.It is presumed that the generated elemental selenium binds to the milky surface layer formed on the surface of the Momordica charantia polysaccharide.The results of electron microscopy confirmed each other,indicating that the elemental selenium reduced by sodium selenite had been successfully combined with the polysaccharide surface of Momordica charantia.4.Momordica charantia-selenium significantly increased the ability to scavenge hydroxyl radicals and DPPH,and it was less effective in removing superoxide anion than Momordica charantia polysaccharides.5.In the study of Momordica charantia polysaccharide-selenium on diabetic mice hypoglycemic effect found that: the middle dose of Momordica charantia polysaccharide-selenium can significantly reduce blood glucose in mice with diabetes and diabetes;can increase INS content in diabetic mice,liver SOD,GSH-Px and CAT activity;can reduce the liver MDA content in diabetic mice;can well control the serum CHO levels in diabetic mice.High dose of Momordica charantia polysaccharide-selenium can control the serum TG content in diabetic mice.6.In the liver and kidney sections of mice in the experimental group of three Momordica charantia polysaccharide-selenium gavage groups,it was shown that diabetic mice treated with selenium polysaccharide can protect liver and kidney while reducing blood glucose.The middle-dose Momordica charantia polysaccharide-selenium had better protective effects on the liver and kidney of diabetic mice.In summary,in the treatment of diabetic mice,selenium polysaccharide can reduce blood glucose,increase the activity of antioxidant enzymes,protect the body's major organs,is a mild polysaccharide drug to relieve symptoms of diabetes.Among them,the medium-dose selenium polysaccharide has the best effect of treating diabetes,and is an agent having potential therapeutic and protective effects.