Optimization Of The Process Of Extraction And Studying On Hypoglycemic Properties Of Polysaccharide From Momordica Charantia

Bitter gourd as cucurbitaceous Momordica plants, is a "medicine food homology" functional food raw material, known as the "medicinal vegetables" said."Dictionary of Chinese medicine" and other records of balsam pear has the effect of clearing the fire, eyesight detoxification, lowering blood sugar, nourishing, Qi[1]. The study also found that, bitter gourd juice, bitter gourd of water extract with antibacterial, adjusting and improving immunity, anti-tumor, inhibit HIV surface activity of. Balsam pear composition is very complex, the water rich, polysaccharide, glycoside and vitamin. At present, the domestic and foreign research of Momordica charantia mainly concentrated in the bitter gourd polypeptide and saponins of lowering blood sugar, antioxidant and immunity, and the other is not clear in balsam pear hypoglycemic active components, such as polysaccharide content of more research is relatively less, polysaccharide and use mostly without purification of the crude polysaccharide, balsam pear origin is not clear, and the action mechanism of Momordica charantia polysaccharides is not comprehensive, the extraction, purification of polysaccharide was studied, And with their extraction of Polysaccharide from Momordica charantia according to design experimental hypoglycemic properties in mice, through experiments to verify the effects of Momordica charantia polysaccharides on diabetic mice hypoglycemic effect and insulin levels, extraction and pharmacodynamic mechanism aimed at the development of Momordica charantia polysaccharide, it obtains the following main results.1.water extraction process of polysaccharide extraction technology, the reference current, improved method of extracting polysaccharide from Momordica charantia and optimize the process to increase the extraction rate of Polysaccharide from Momordica charantia and balsam pear percent content of polysaccharide. Based on the solubility and the specific components of Momordica charantia polysaccharide analysis and design steps of the experiment, the optimum extraction method, The results show that:the extraction rate is relatively better by double enzyme hydrolysis method of pectinase and cellulase, the best process optimization:time is1hours, water ratio is10:1, extracting temperature was80℃, the extraction rate was3.3%, the content of polysaccharide was23.2%, than the hot water extraction, ultrasonic extraction, enzymatic extraction method were increased by9.8%,15.3%and9.5%.2.1n the choice of good method to choose between three types of bitter gourd, bitter gourd, bitter gourd, short rounded oblong strip balsam pear, determine the content of what kind of balsam pear Momordica polysaccharide relatively. The results showed that the strip of balsam pear; short round and oblong melon is good, so choose strip of balsam pear. Then the strip of Momordica charantia further classification, divided into white (old) strip of balsam pear and green (tender) strip of balsam pear were used in the experiment, the result shows that the (old) strip of balsam pear is green (tender) strip of balsam pear is better, so eventually determine the final selection for white (old) strip of balsam pear.3.Identification and purification of polysaccharide crude product and purification of polysaccharide composition. Momordica polysaccharide purification is mainly divided into two steps:the first step of purification; method is first to Momordica polysaccharide in80℃water bath dissolution, supernatant from experience, removal of protein by Sevage method, H2O2method in addition to the pigment, water concentration, ethanol precipitation, dialysis, freeze drying to obtain polysaccharide purification. The second step depth purification; method is the preliminary purification of Momordica charantia polysaccharides by DEAE-32ion chromatography, using0.1mol/L NaCI solution for elution, collecting the corresponding peak group, after concentration, distilled water dialysis, refined polysaccharide freeze-drying got. Methods the identification of purified polysaccharide component is to determine polysaccharide structure, corresponding functional groups and protein combining with infrared chromatography and ultraviolet chromatography, and then purified polysaccharide acid hydrolysis, by thin layer chromatography, can determine its monosaccharide composition, further more refined balsam pear sugar was hydrolyzed and acetylated GC-MS method was used to analyze the monosaccharide composition, to determine the main composition and proportion of Momordica charantia polysaccharides in.4.Effects of Momordica charantia polysaccharides on diabetic mice hypoglycemic effect and insulin levels, method is to use the Kunming mice with streptozotocin diabetic model of preparation and use of Momordica charantia polysaccharides extract treatment, observe the effect of hypoglycemic effect of Momordica charantia polysaccharides and the insulin level in the course of treatment. Results:compared with the untreated diabetic mice, Momordica charantia polysaccharides treatment significantly reduced the mice blood glucose, insulin levels were significantly up-regulated, insulin resistance index increased significantly, the insulin sensitivity index decreased significantly.The innovation of this paper lies in:(1) to establish the method and condition of extracting balsam pear enzyme hydrolysis of Polysaccharide from Momordica charantia polysaccharides, determined the best sources from balsam pear Momordica polysaccharide;(2) the polysaccharide was purified by methods to choose, and the main components of Momordica charantia polysaccharides the composition, proportion and structure;(3) and used to extract polysaccharide on diabetic mice were used in the experiment, determined the polysaccharide has significant hypoglycemic effect in diabetic mice, affect the secretion of insulin, the insulin resistance index increased significantly, insulin sensitivity index decreased significantly.