The Study Of Expression And Role Of Myosin Interaction G Protein Exchange Factor In Epithelial Ovarian Cancer

Background: Ovarian cancer is one of the three most common malignancies in female genitals,and the fatality rate ranks first in gynecologic malignancy.Epithelial ovarian cancer accounts for 85%-90% of ovarian cancer and is the most common histological type.A large number of experimental studies have shown that the Rho signaling pathway plays an important role in tumorigenesis and the molecular mechanism of tumor progression.Recent studies have shown that the dysregulated expression of myosins interact G protein exchange factor(MyoGEF)promotes breast cancer metastasis through regulated RhoA.However,the expression and role of MyoGEF in epithelial ovarian cancer remains unclear.Methods: The expression of MyoGEF in epithelial ovarian cancer tissues was detected by immunohistochemistry.The correlation of MyoGEF expression with epithelial ovarian cancer tumor size,metastasis,differentiation,and postoperative survival was analyzed statistically.The effect of MyoGEF on metastasis and invasiveness of ovarian cancer cells was examined using a transwell assay.Results: The results of immunohistochemistry showed that MyoGEF protein expression in epithelial ovarian cancer tissue was significantly higher than that of normal ovarian tissue in the control group.The level of MyoGEF protein expression was significantly correlated with the degree of tumor differentiation,metastasis and prognosis in the patient's clinical data.By knocking down the expression levels of MyoGEF in ovarian cancer cells A2780 and SKOV3,it was found that the metastatic and invasive ability of ovarian cancer cells was significantly inhibited.Conclusion: MyoGEF was overexpressed in epithelial ovarian cancer tissues.MyoGEF promoted the progression of ovarian cancer by regulating the metastasis of ovarian cancer.MyoGEF might serve as a molecular target for epithelial ovarian cancer diagnosis and prognosis.