Regulation Of Bushen Jiedu Prescription On Dendritic Cells Under Radiation Conditions

ObjectiveTo study the damage of bone marrow dendritic cells(DC)in mice exposed to low,medium and high radiation doses.To observe the protective effect of Bushen Jiedu Prescription on DC,and to compare the effects of combination of Bushen Jiedu prescription prevention and treatment group(BPT)?Bushen Jiedu prescription prevention group(BP)on DC damage induced by different doses of radiation.The protective effect and regulatory mechanism of Bushen Jiedu prescription on DC after radiation were systematically studied.Methods270 mice were divided into 3 groups by random number table.Each group of mice was randomly divided into 5 groups(18 mice per group).They were control group(CG),radiation model group(RG),amifostine group(AG),BPT and BP.BP and BPT were given orally at 9:00 a.m.every day.CG,RG and AG were given saline of equal volume.The gastric volume was 0.25 mI.On the 11 th day after the start of the experiment,three groups of mice were irradiated with different doses of Cogamma-ray at 2Gy,4Gy and 6Gy respectively.On the 1st,7th and 14 th days after irradiation,six mice were selected from each group randomly.The percentage of positive cells of MHC-II molecule in bone marrow dendritic cells of mice was detected by flow cytometry,and the four main co-stimulator molecules on the surface of DC,CD80,CD83,CD86 and CD284,were detected by flow cytometry.The percentage of positive cells and the maturation and activation of DC were observed by immunofluorescence.Results(1)Flow cytometry was used to detect the expression rate of DC positive cells:(1)2Gy low dose radiation: on the 7th day after radiation,the percentage of MCH-II + Molecular cells and CD80,CD83,CD86 and CD284 positive cells of BPT increased,showing a strong protective effect,followed by BP.On the 14 th day after irradiation,the expression rate of BP positive cells decreased significantly,while BPT only showed a slight decrease.(2)4 Gy and 6 Gy dose radiation: The expression rate of BP positive cells was higher than that of BPT,and the protective effect of BP and BPT on dendritic cells was weaker than that of the same group under 2 Gy radiation.(2)The maturation and activation of DC were observed by immunofluorescence: Under different radiation doses,CG bone marrow cells can differentiate into mature DC cells;while radiation inhibits the ability of bone marrow cells to differentiate and mature into DC cells.With the increase of radiation doses,it can be observed that the ability of differentiation and maturation of DC cells in RG decreases gradually;BPT and BP can better reverse the ability of differentiation and maturation of bone marrow cells induced by radiation,AG showed protective effect only on the first day after radiation.ConclusionsBushen Jiedu Prescription can promote DC maturation and activation,and enhance the body's protection against radiation:(1)Under different radiation doses,the expression rates of HC-II molecules in BPT group and BP have increased to varying degrees,indicating that Bushen Jiedu Prescription can promote DC maturation.(2)The expression rates of CD80,CD83,CD86 and CD284 on the surface of DC were increased,which indicated that Bushen Jiedu Prescription could promote the maturation and activation of DC.(3)Cell immunofluorescence was used to observe the maturation and activation of DC.BPT and BP did not show protective effect on the first day after radiation.BPT and BP on the 7th,14 th day after radiation could better reverse the differentiation and maturation ability of bone marrow cells to DC cells induced by radiation.(4)The protective effect of BPT is better than that of BP under low dose radiation of 2Gy,and that of BP under medium and high dose radiation of 4Gy,6Gy is better than that of BPT.