The Levels Of VCP And Alpha-fodrin In Peripheral Blood Of Patients With Primary Glaucoma

BackgroundGlaucoma is the second leading cause of blindness following cataracts,and its blindness is irreversible,which will pose even greater challenges to global public health.Glaucoma has so far no exact method to cure it,and its pathogenesis is not yet clear.Previous studies have shown that autoimmune mechanisms may be involved in the progression of glaucoma patients.Elevated levels of VCP and ?-fodrin are found in glaucoma experimental animal models,but changes in VCP and ?-fodrin in glaucoma populations and their diagnostic value for disease still not clear.ObjectiveWe examined the differences in VCP and ?-fodrin in peripheral blood of patients with different types of primary glaucoma and normal subjects,and evaluated the diagnostic value of each index in glaucoma.MethodsWe collected 80 patients with glaucoma who were first diagnosed at the Fifth Affiliated Hospital of Zhengzhou University and the First Affiliated Hospital ofZhengzhou University from January 2015 to September 2017,including 40 patients with primary angle-closure glaucoma.Forty patients with primary open angle glaucoma group,and 40 healthy subjects from Zhengzhou University's Fifth Affiliated Hospital were collected as control group.VCP and ?-fodrin in peripheral blood of each group were detected by enzyme-linked immunosorbent assay.The content of the receiver was plotted against the indicators,and the area under the curve was calculated.Multivariate logistic regression was used to analyze the risk factors of glaucoma patients.Data analysis was performed using SPSS 21.0 software.The quantitative results were expressed by?x ±S,and the difference was statistically significant when P < 0.05 was defined.Results1.The VCP content in the POAG group,PACG group and control group respectivelywere(124.866 ± 34.858)ng·L-1,(94.143 ± 28.970)ng·L-1 and(44.707 ± 8.892)ng·L-1,There was a statistically significant difference in the content of VCP between POAG group,PACG group and normal control group(P<0.05).2.The ?-fodrin content in the POAG group,PACG group and control grouprespectively were(0.531 ± 0.306)?g·L-1,(0.399 ± 0.213)?g·L-1 and(0.304 ± 0.214)?g·L-1,There was a statistically significant difference in the content of ?-fodrin between POAG group,PACG group and the normal control group(P<0.05).3.The area analysis under the receiver operating characteristic curve showed that theVCP was 0.754(95% CI 0.646~0.862,P<0.001),?-fodrin was 0.610(95% CI 0.486~0.733,P = 0.091),Prompt VCP has certain diagnostic performance.4.Multivariate logistic regression analysis suggested that VCP was an independentrisk factor for the onset of POAG patients(OR=9.134,P<0.01).Conclusions1.The levels of VCP and ?-fodrin in POAG and PACG patients were higher thanthose in normal subjects,indicating that immune factors were involved in thepathogenesis of POAG and PACG.2.VCP is an independent risk factor for the onset of POAG patients.