Research On The Commercial Specification Grades And Quality Of Huai Flos Chrysanthemum

Objective:Through the survey of the production and market investigation of Huai flos chrysanthemum medicinal materials and decoction pieces,based on the traditional classification methods of Huai flos chrysanthemum medicinal materials,combined with modern scientific research technology,to study the samples specification hierarchy and quality characteristics of Huai flos chrysanthemum.Meteods:First of all,use the methods such as shape,size,color,texture and smell to describe the traits of the collected 48 samples of Huai flos chrysanthemum,mainly according to the Huai flos chrysanthemum varieties,size of the sample classification,and then get 134samples;Secondly,conduct the trait identification,microscopic identification and thin layer identificationspecifications of different commodities of Huai flos chrysanthemum according to the 2015 edition of China pharmacopoeia,using traditional Chinese medicine DNA barcode molecular to identy different commodities of Huai flos chrysanthemum;To check the water of different commodities of Huai flos chrysanthemum according to the 2015edition of China pharmacopoeia,using inductively coupled plasma mass spectrometry?ICP-MS?to check Pb,Cr,As,Hg,Cu five kinds of heavy metal elements in limited inspections of different commodities of Huai flos chrysanthemum;To Apply high performance liquid chromatography?HPLC?with different product specifications and grades of chlorogenic acid in Huai flos chrysanthemum,mignonette glycosides,3,5-O-2 coffee acyl quinic acid ingredients in a three indexes,content determination,application of ultraviolet-visible spectrophotometry?UV?specifications and grades of different commodities Huai flos chrysanthemum for content determination of total flavonoids in the steam distillation is adopted to different specifications and grades commodity Huai flos chrysanthemum volatile oil content determination,and use variance analysis in SPSS statistical software to analyze five chemical indicators;Finally,by gas chromatography tandem mass spectrometry?GC-MS?combined with NIST standard MS library to analyze volatile oil components,and application of partial least squares of chemometrics-validated discriminant analysis?PLS-DA?;By the method of HPLC,to study on fingerprint Huai flos chrysanthemum water-soluble chemical composition.Results:Eventually Huai flos chrysanthemum samples collected into cv.Dabai ju?cv.Xiaobai ju?cv.Xiaohuang ju and cv.Zhenzhu ju four goods specifications,including cv.Dabai ju,cv.Xiaobai ju?cv.Xiaohuang ju was divided into three levels,cv.Zhenzhu ju is divided into two levels.Microscopic identification that the Pollen grains,T-shaped hairs,glandular hairs,calcium oxalate crystal clusters,bracts epidermis,anther wall cells,medicine diaphragmatic basal cells in different commodities Huai flos chrysanthemum.Thin layer identification,according to the results of cv.Dabai ju?cv.Xiaobai ju?cv.Xiaohuang ju and cv.Zhenzhu ju chromatography to the control of the chromatography and chlorogenic acid reference substance chromatography corresponding position,all show the same color of the fluorescent spots.The water in Each sample is in accordance with Chinese pharmacopoeia standards.Molecular identification results show that the ITS2 DNA barcode cannot be accurately identify four Huai flos chrysanthemum commodity specification.Heavy metal results showed that the white chrysanthemum 5,8,9,10 and 22 samples of feverfew Pb is greater than 5.0 mg·kg^-1,four specifications Cd were greater than 0.3 mg·kg^-1.On the chemical composition,analysis of variance,according to the results of different commodities specifications,at the same level between different specifications with a significant difference between the same specifications and there was no significant difference between different grades.Volatile oil components GC-MS analysis,a total of 49 compounds identified,including common composition for 32,mainly for the single terpenoids and sesquiterpenoids and oxygen-containing derivatives,partial least squares discriminant analysis results will be the sample is divided into three categories,cv.Xiaobai ju and cv.Xiaohuang ju together,from cv.Dabai juand cv.Zhenzhu ju together separately.Respectively,set up cv.Dabai ju?cv.Xiaobai ju?cv.Xiaohuang ju and cv.Zhenzhu ju water soluble component fingerprint and huai chrysanthemum water-soluble ingredients fingerprint.Conclusion:This method can provide a reference for Huai flos chrysanthemum from different commercial grades.