The Study Of Cortical Angiogenesis After Photothrombotic Ischemia In Rats Cortex Based On OCT Imaging

Stroke is one of the three major threats to human health.Ischemic stroke is characterized by high morbidity and disability,which seriously affects the quality of life of patients.Vascular regeneration and remodeling are complex four-dimensional space-time processes in the brain injury area caused by cerebral ischemia.Understanding its dynamic changes and characteristics of regenerated blood vessels plays an important role in the treatment and rehabilitation of brain injury.The regulation model of this process is one of the hot topics in recent years.This paper aims to perform cortical angiography in normal and focal cerebral ischemia rats by optical coherence tomography(OCT).Cortical angiography was performed in rats to observe the changes of cortical blood vessels after ischemic injury in real time,revealing the regularity of angiogenesis after ischemic stimulation,and provide experimental technical basis for future investigation of stroke injury and diagnosis and treatment.The treatment of sexual diseases provides theoretical guidance and new methods.This paper created an animal light window model for in vivo,minimally invasive,real-time continuous observation of changes in rat cortical ischemia.Based on the photothrombotic ischemia in rat cortex,we recorded the thrombosis formation and the damage of the capillary networks using optical coherence tomography(OCT).At the same time,the recanalization and recovery of blocked vessels,the regeneration process of peripheral vessels and small vessels all were observed continuously from 0 to 14 days after the ischemia.On this basis,the vascular damage and recovery of rat ischemic cortex were comprehensively evaluated by analyzing the blood vessel diameter,blood vessel area density and blood flow parameters of different lesions,combined with immunopathology.The results of this study showed that(1)the formation of light plugs in cortical blood vessels was a process of gradual spread,from the strongest point of laser irradiation to a region that spread around this point.(2)Within 24 to 48 hours of ischemia,the area of brain injury was the largest,and the blocked large blood vessels were spontaneously recanalized.The superficial microvascular was reperfused with the neovascularization from the peripheral to the core.After 14 days,the microvessel density increased compared with the pre-ischemic base value,but not recovered after the deep blood vessels were damaged.(3)Compared with the male group,the vascular recovery rate of the ischemic region of the female rats was faster.In this study,theoretical and methodological innovations in model making and subsequent treatment are conducive to uninterrupted long-term observation experiments,such as optical coherence tomography imaging combined with well-designed photothrombotic stroke model and chronic optical window.Monitoring the cerebral cortical angiogenesis from two-dimensional/three-dimensional angles is conducive to uninterrupted long-term observation experiments,laying the foundation for in-depth quantitative study of the dynamics of occlusion of blood vessels and peripheral capillaries during cerebral ischemia recovery,and is expected to be in the injured area.We try to provide a solid theoretical basis for rehabilitation and treatment after stroke,and theoretical guidance and new methods for the treatment of cerebral ischemic diseases.