| Objective:The effect of different decoction time of Pueraria lobata on the content of puerarin in Shengma Gegen Decoction was detected by high performance liquid phase method.The animal model of cold-related infection was induced by pyrogenic method,acute inflammation method,pain-causing method and cough method.Coking time oppsite the antipretic effct of radx soup of areries and veins anti-inflaatory effct and anlgesic action,antitsive action and effct of immne fuction.To explore the best boiling time of Pueraria lobata in Shengma Gegen Decoction.Method:High-performance liquid phase method was used to detect the effect of different decoction time of Pueraria lobata on puerarin content in C.sinensis.Chromatographic conditions:Agilet 5 TC-C18 HPLC colun(250 x 4.6 mm,5 mirons),moile phase:methnol-wter captred the(5),30°C colun,flw rat of 1 mL/min,detecton welength of250 nm,injection volume 10?L.Using 15%yeast suspension pyrolysis metod,egg wite-indced acte inflamation,hot plte pan-causng metod,25%amoia-indced cogh metod to replcte the cld-relted anmal mdl,observe the body temperature of the cold-related model animal,the toe swelling rate,the pain threshold,Cough latency,cough frequency,spleen coefficient,thymus coefficient,and immune factor(IL-6)levels.Result:High-performance liquid phase detection showed that puerarin showed a good linear relationship with the peak area in the range of 0.02~0.12mg/mL(r~2=0.9996);No.1decoction(Guigen decoction 20min group)in the test solution The content of puerarin was30.0 mg/g;the puerarin content of the test solution was 34.8 mg/g in the No.2 decoction(25min group);the diarrhea in the test solution was prepared in the No.3 decoction(the pueraria decoction for 30 min)The content of puerarin was 38.4 mg/g;the amount of puerarin in the test solution was 43.6 mg/g in the decoction of No.4(35 min in puerarin decoction);the puerarin in the test solution was prepared in the decoction of No.5(40 min in pueraria decoction)The content of the pigment was 42.4mg/g;the No.6 decoction(the root of pueraria decoction for 45min)was used for the test solution solution of 41.2mg/g;the No.7 decoction(the Kudzu root decoction 50min group)was used in the test solution.The content of puerarin was 39.6 mg/g;the content of puerarin in the test solution of No.8 decoction(Kudzu decoction 55 min group)was 36.4 mg/g;the supply of No.9 decoction(Jigen decoction 60min group)The content of puerarin in the test solution was 36.0 mg/g.Body tempertre test,coprd with the blnk cotrol grop,the body temperature of the model group was significantly increased at 1,2.5,3,4,5,6,7,8h aftr injeton of 15%yeas supesion(P<0.05 or P<0.01).Comared ith the odel grop,te positve cotrol grup ad te positve cotrol grup 2 decreased significantly at 1,2.5,3,4,5,6,7,8h after injection of 15%yeast suspension(P<0.01).;the body temperature of the group was 30,35,40 min after injecting 15%yeast suspension for2.5,3,4,5,6,7,8h,the body temperature was significantly decreased(P<0.05 or P<0.01);The body temperature of the 25 min group was significantly decreased at 4,5,6,7h after injection of 15%yeast suspension(P<0.05).The toe swelling rate was measured.Compared with the blank control group,the toe swelling rate was significantly increased in the model group at0.5,1,2,3,4h afer injeton of te egg whte liqid(P<0.01).Comped with the mel grup,the potive rate was positive.In the control group 1,the positive control group 2,the radix decoction30,35,40,45,50,55,60min group,0.5,1,2,3h after injection of egg white,the toe swelling rate as sinifianly reued(P<0.05 or P<0.01 or P<0.01);the swling rate of te to wa sinifiantly deceasd at 0.5,1,2h after the injection of egg white for 20,25min(P<0.05).Pain threshold detection,compared with the blank control group,the positive control group 1,the positive control group 2,Pueraria lobata 30,35,40min group pain threshold significantly increased(P<0.05).Cough latency and cough frequency were compared.Compared with the blank control group,the cough latency of the positive control group,the positive control group 2,the radix decoction for 30,35,40,45 min was significantly increased(P<0.05 or P<0.01).The number of coughs in the control group,the positive control group 2,the radix decoction for 35min,and te 40 mn grup was sgnficntly lowr(P<0.05).The slen and tyus index er cmard.Compared with the blank control group,the spleen coefficient of the positive control group 1,the positive control group 2,the radix decoction 30,35,40,45,50,55min group was significantly increased(P<0.05 or P<0.01).The positive control group,the positive control group 2,the radix puerariae for 30,35,40,45,50min,the thymus coefficient of the group was significantly increased(P<0.05 or P<0.01).Enye-lied imunsrbet assy(ELISA),comard wih te blank control group,the positive control group 1,the positive control group 2,Pueraria lobata 25,30,35,40,45min group IL-6 levels were significantly increased(P<0.05 or P<0.01or P<0.001).Conclusion:On the decctin praces,puerarin is decocted for 35 minutes,then mixed with other medicinal liquids in Shengma Puerarin Decoction.Finally,the content of Puerarin in Shengma Puerarin Decoction will increase significantly,and it has obvious alleviating effect on the disease symptoms in related animals.Shengma Gegen Decoction has a wide range of clinical applications.In the specific research and use,it is necessary to further diagnose and determine the best method of use in the light of clinical conditions. |
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