Pueraria Chemical And Biological Active Substances, Separation, Purification

Pueraria Lobata is a kind of leguminous plant. It distributes in most districts of China and grows in warm and moist surroundings. It can be divided into Pueraria lobata (wild) Ohwi and Pueraria thomsonii Benth. Pueraria Thomoni Benth is rich of starch, which is a kind of important source of food material and additive. Pueraria isoflavones are blieved to have significant pharmaceutical functions to human health.Isoflavones are a kind of scarce raw materials of medicine and chemical industries. Radix Pueraria contains rich isoflavones. Pueraria Lobata is a kind of rapidly growing plant, which is suitable for extensive implantation. Since the rich resources of Pueraria in our country, Pueraria flavonoids are eagerly needed in either domestic or the international markets, while the extraction and prification techniques are needed to be improved at present. We report here the studies on the extraction and purification of the isoflavones from Radix Pueraria and preparation of pury Puerarin.As the results:1. In the course of Pueraria isoflavones extraction, pH value, temperature, solvent and consistency of solvent affected the macerating efficiency. The bad efficiency was monitored when pH value of solution was lower than 4 or higher than 8. As for the temperature, it indicated that the higher temperature was, the higher macerating efficiency was. However, the high temperature over 85Cmay destroy the structure of isoflavones. By comprehensive corporations, it showed that extract with alcohol was better than that with water. The extract was difficult to be filtered when the content of alcohol was lower than 50%, and lower macerating efficiency when the content of alcohol was higher than 95%. The suitable consistency of alcohol was about 70-80%.2. By the orthogonal experiments, the optimum macerating conditions we acquired were pulverized and powdered the samples before extraction, 70% alcohol, 85 C, pH6-8, Ihr, 2 times, solid/liquid 1: 10. In such conditions, the yield, the content of products and the macerating efficiency were 19.85%, 42.00%, and 92.0% respectively.3. By hydrolyzed of alcohol extract with enzymes or 5% HC1 in two-liquid-system, the extract efficiency was monitored increased a little. After extracted for four times, the Pueraria isoflavones was almost completely extracted. The yields and the purity were up to 50.86% and 60.85% respectively.4. The crystallization conditions for Pueraria isoflavones were optimized to be 10C , 95% consistency of solvent, solid/liquid 1: 1.5. The yield and the purity were 36.5% and 79.2% respectively.5. The poliamid column chromatography was shown to be better than Silica gel and Al2O3 for the isolation of Puerarin, and both the HPLC and TLC could be settled to monitor the elutes from column chromatography. By the poliamidcolumn chromatography, the content of Puerarin in the elute was up to 95%.6. We designed a preparation process for Puerarin.7. We carried out the spectral analyses (MS, IR, 1HNMR, 13CNMR) of our product. Our product was conformed to be Puerarin by comparing the charts and data with that of the standard atlas and the document data.