Studies On Preparation Technology And Quality Standard Of Ginsenoside Niosome

Objective:Panax is a dry root and rhizome of Panax ginseng C.A.Meyer,which has a wide range of p Harmacological and biological activities.The extract extracted from roots and stems contains ginseng total saponins and has good antioxidant activity.However,the skin permeability of ginseng total saponin is not so good that it does not fully exert its efficacy.Therefore,through the new technology of ginseng total saponin liposome,the application range of ginseng total saponins has been expanded and played a role.Methods: The preparation of liposome was carried out by ethanol injection method;the liposome and free drug were separated by high-speed centrifugation-filtration method and a feasible method for determining the encapsulation efficiency was established;in order to measure the content of total ginsenoside in ginseng,the selection was carried out.High-p Hase liquid chromatograp Hy;using single factor investigation and orthogonal test to optimize the preparation conditions of ginseng total saponin liposome;preparation of ginseng total saponin liposome freeze-dried powder by freeze-drying method,and quality Standard studies,including morp Hological studies,particle size and zeta potential measurements,and DSC analysis;in vitro skin permeability experiments on ginseng total saponin liposomes.Results:The in vitro analysis method of ginseng total saponins is relatively stable and feasible.The optimal preparation process of ginseng total saponin liposome preparation is as follows: stirring temperature is 65?,the ratio of absolute ethanol to PBS is 1:10,and the drug loading is 60 mg;three batches of samples are prepared according to the optimal preparation process,and the average encapsulation efficiency is measured.64.35%;ginseng total saponin liposome lyop Hilized powder is prepared by adding 0.1 g of mannitol as a protective agent per 10 ml of ginseng total saponin liposome suspension,that is,obtaining a loose lyop Hilized powder.And the encapsulation efficiency was measured to be60.45%.The ginseng total saponin liposome had a particle size of 60.93 nm,a PDI of 0.086,a zeta potential of-35.5 mv,a particle size of 64.38 nm,a PDI of 0.245,and a zeta potential of-38.1 mv before lyop Hilization.And the encapsulation rate does not change much.According to the results of the differential scanning calorimetry,there is an interaction between the total ginsenosides and the liposome,and the drug is encapsulated therein by the liposome,rather than simply p Hysical mixing.Conclusion: The single-factor investigation and orthogonal test confirmed the preparation process of the relatively stable ginsenoside liposome,and the repeatability was relatively good.In the quality standard study,each index met the liposome requirements;In the skin permeability test,it was confirmed that liposome can enhance the skin permeability of ginseng total saponin.