Comparison Of The Effect And Mechanism Of Transplantation Of Bone Marrow Mesenchymal Stem Cells And Cardiac Sca1-Positive Stem Cells For Myocardial Infarction

ObjectiveTo investigate the therapeutic effect of bone marrow mesenchymal stem cell(b MSC)and cardiac-derived Sca1 positive stem cell in mice acute myocardial infarction model.MethodsBone marrow mesenchymal stem cells were isolated from the femur bone marrow of adult C57BL/C mice,and Sca1 positive stem cells were harvested from the heart by flow cytometry.Twenty-four male C57BL/C mice were divided into four groups: false surgery group,control group,bone marrow b MSC treated group and Sca1 positive cells treated group.After anesthesia,the mice thorax was opened and the left anterior descending coronary artery(LAD)was ligated to establish the acute myocardial infarction model.After successful LAD ligation,cells were injected into the infarcted myocardium.Four weeks later,cardiac ultrasound was used to observe heart function: the left ventricular ejection fraction(LVEF),left ventricular short axis fractional shortening(LVFS),left ventricular(LV)end systolic volume and LV end dilated volume.In addition,quantitive PCR was used to evaluate the RNA expression of VEGFa,VEGFb and in cultured cells.ResultsThe cultured b MSC adhered to the dish and showed a spindle shape.The positive rate of Sca1 positive stem cells was about 18 %,and they also adhered to the dish while showed a short shuttle-like shape.Compared with Sca1 positive stem cell therapy,b MSC treatment increased the survival rate of mice after myocardial infarction.After 4 weeks of cell transplantation,LVEF and LVFS in the b MSC treated group were significantly higher than those in the heart-derived Sca1-positive stem cells,and significantly inhibited the expansion of left ventricular end-systolic volume and left ventricular end-diastolic volume after myocardial infarction,and inhibited myocardial remodeling.The expression of VEGFa and VEGFb is significantly high in b MSC.ConclusionsBoth bone marrow-derived MSC and Cardiac-derived Sca1 positive stem cells were benefit to the improvement of cardiac function after infarction,and b MSC are superior to improve LVEF and inhibit enlargement of left ventricular after myocardial infarction in mice,this may be caused by the high expression of VEGF in b MSC.