Observe Protective Effects And Mechanism Of Qufengzhitongsan On Gouty Nephropathy And Acute Gouty Arthritis Rat

Objective:Gout is a complex metabolic disease with increasing incidence.It has been clear that gout is related to the deposition of uric acid in limb joints and connective tissue.The protective effect and mechanism of QFZTS on gouty nephropathy and acute gouty arthritis model rats were studied in this experiment.To provide new target sites and therapeutic methods for clinical treatment of gouty nephropathy and acute gouty arthritis.Methods:1Study on the effect and mechanism of QFZTS on gouty nephropathy rat model:To establishment the gouty nephropathy model,the rats in the normal group were given pure water of equal volume,the others group were given the suspension of yeast with adenine(yeast 10 g·kg^-1,adenine 100mg·kg^-1)by intragastric perfusion for 19 days;Starting from the 20th day,the rats in the normal and model group were given pure water,the others group were given corresponding liquid by intragastric perfusion of 1 mL·100g^-1.The dosage is allopurinol group:0.01 g·kg^-1,QFZTS high,middle,low dose group:1.6g·kg^-1,3.2g·kg^-1,6.4 g·kg^-1,lasting 20 days.Serum uric acid?SUA?,serum creatinine?SCr?,blood urea nitrogen?BUN?,Superoxide dismutase?SOD?,Malonaldehyde?MDA?,interleukin-1??IL-1??,interleukin-6?IL-6?,Tumor necrosis factor-??TNF-??,transforming growth factor-?1?TGF-?1?,renal index?RI?of rats in each group were examined.The pathological changes in renal tissue sections of rats in each group were observed.The expressions of Nucleotide-bound oligomerized domain-like receptors3?NLRP3?,Cysteinyl aspartate specific proteinase-1?Caspase-1?,IL-1?messenger RNA in kidney tissues were detected.2Study on the effect and mechanism of QFZTS on acute gouty arthritis rat model:Rats in each group were intragastric perfusion once a day.The blank group and model group were given pure water of equal volume,and the other groups were given corresponding solution,lasting 7 days.On the fourth day,after 1 hour of gastric perfusion,acute gouty arthritis rat model was established by MSU injection in ankle joint cavity.The inflammation,dysfunction and joint swelling index degree was measured after making model at 0,4,10,24,48 and72h.The contents of WBC,SOD,MDA,NEUT,IL-1?,IL-6,TNF-?and TGF-?1 of rats were measured after 72h.The pathological changes in the synovial tissue of ankle joint were observed.The expressions of NLRP3,Caspase-1,IL-1?messenger RNA in the soft tissue of ankle joint were detected.Results:1Compared with model group,the levels of SUA,SCr,BUN,MDA,IL-1?,IL-6,TNF-?,TGF-?1 and RI of rats with gouty nephropathy could be obviously decreased after QFZTS treatment.The levels of SOD could be increased.Tubular dilatation and interstitial fibrosis can ben improved.Inflammatory cell infiltrationcan be reduced.The expressions of NLRP3,Caspase-1,IL-1?messenger RNA in kidney tissues were down-regulated.2Compared with model group,the inflammation,dysfunction and joint swelling index degree could be reduced after QFZTS treatment.The levels of WBC,SOD,MDA,NEUT,IL-1?,IL-6,TNF-?and TGF-?1 could be obviously decreased after QFZTS treatment.The levels of SOD could be increased.Tubular dilatation and interstitial fibrosis could be improved.Inflammatory cell infiltration could be reduced.The expressions of NLRP3,Caspase-1,IL-1?messenger RNA in the soft tissue of ankle joint were down-regulated.Conclusion:QFZTS has protective effect on renal function damage and acute gouty arthritis in rats with gouty nephropathy.Its mechanism may be through down-regulating the expression of key molecular genes in NLRP3-Caspase-1-IL-1?inflammatory pathway,and then reducing the release of inflammatory factors,such as IL-1?,IL-6,TNF-?and TGF-?1,to delay the process of inflammation.It may also be related to the inhibition of oxidative stress.