Expression Of SVAP-1 And Its Clinical Significance In Serum Of Patients With Chronic Hepatitis B

In the clinical diagnosis and treatment,chronic hepatitis B(CHB)is a common disease in digestive medicine and infectious department.CHB is a common and infectious disease in clinic.Because of its special clinical characteristics,it has a great impact on the health of patients and others.There are about 350 million CHB patients in the world.In the investigation of infection status in China,the carrier rate of hepatitis B surface antigen(HBsAg)was 7.18%in the population aged 1-59 years.When CHB is not well controlled,it tends to develop into cirrhosis,even to the stage of liver cancer,which seriously affects the treatment and prognosis of patients.This brings heavy mental and financial burden to patients and their families.Moreover,CHB patients will be the source of infection for a long time,and have an important impact on the health of others.Therefore,the treatment and prevention of CHB is of great significance.Vascular adhesion protein-1 is an endotheliocyte adhesion molecule.Vascular endothelial cells,adipocytes and smooth muscle cells are the main secretory cells.Under the action of physiological shear stress,it participates in the migration,exudation and migration of inflammatory cells such as white blood cells and granulocytes,showing the pro-inflammatory characteristics.VAP-1 was hydrolyzed by protease,it can form soluble vascular adhesion protein-1.It plays a functional role in human blood circulation.The aim of this study was to analyze the relationship between serum sVAP-1 level and hepatitis B virus DNA(HBV DNA),Alanine aminotransferase(ALT),Aspartate aminotransferase(AST),?-glutamyl transferase(?-GT),Total bilirubin(TBil),Albumin(Alb),hyaluronic acid(HA)level in patients with chronic hepatitis B.To investigate the role of sVAP-1 as an inflammatory factor in the pathogenesis of CHB.We hope to provide new ideas for the immunotherapy of CHB.Objective The original intention of this research design was to analyze the relationship between human serum soluble vascular adhesion protein-1(sVAP-1)level and hyaluronicacid(HA),HBVDNAandliverfunctionrelatedindicators(Alb/ALT/AST/Tbil/gamma-GT)in patients with CHB.To investigate the correlation between serum sVAP-1 level and liver fibrosis and inflammation,HBV-DNA replication in CHB patients.Methods Thirty-two patients with CHB were enrolled in this study as experimental group.The patients were divided into two groups according to their serum HBV DNA level.Including:20 cases of HBVDNA positive group(CHB1 group),12 cases of HBVDNA negative group(CHB2 group).Forty healthy persons were enrolled in the same hospital during the same period as control group(NC group).The cubital venous blood of all the subjects was collected by heparin sodium anticoagulant vacuum collection tube in the morning(12 hours after fasting overnight)for3-5 ml.After centrifugation with 3000rpm/min for 10 minutes,collect supernatant and separate it into freezing tube.Refrigerator storage for inspection at-70?.Serum sVAP-1and HA were detected by enzyme-linked immunosorbent assay(ELISA).Normal distribution data are expressed in the form of mean±standard deviation,t-test or one-way ANOVA were used for comparison between groups.The skewed distribution data are represented by the median,wilcoxon rank sum test was used for comparison between groups.Spearman test was used to analyze the correlation between indicators.Results(1)Serum sVAP-1 values in CHB group and NC group were(19423.01±3743.49),(1544.52±1058.58)ng/ml,respectively.The difference between the two groups was statistically significant.(t=38.996,P<0.001).(2)The serum HA values in CHB and NC groups were(3721.53±441.58),(383.16±118.21)pg/ml,respectively.The difference between the two groups was statistically significant.(t=61.971,P<0.001).(3)The serum sVAP-1 levels in CHB1 group,CHB2 group and NC group were(20008.61±3374.61),(18513.92±4335.47),(1544.52±1058.58)ng/ml,respectively.There was significant difference between the three groups(P<0.05).The data between groups were compared in pairs,the level of sVAP-1 in CHB1 group was higher than that in CHB2 group and NC group,and the difference was statistically significant(P<0.05).The level of sVAP-1 in CHB2 group was higher than that in NC group(P<0.05).(4)The serum HA levels in CHB1 group,CHB2 group and NC group were(3809.76±318.70),(3574.50±579.95),(383.16±118.21)pg/ml,respectively.There was significant difference between the three groups(P<0.05).The data between groups were compared in pairs,the level of HA in CHB1 group was higher than that in CHB2 group and NC group(P<0.05).The HA level in CHB2 group was higher than that in NC group(P<0.05).(5)The results of serum sVAP-1 levels in CHB1 group with different viral levels(low,medium and high viral replication levels)and NC group were(20329.03±3394.59),(19386.05±4147.91),(20518.28±2570.81),(1544.52±1058.58)ng/ml,respectively.The serum sVAP-1 levels of replication group with different viral levels were significantly different from those of healthy control group(P<0.001).But serum sVAP-1 levels were compared between replication groups with different viral levels,the experimental data have no statistical significance(P>0.05).(6)Serum sVAP-1 levels in CHB patients with normal ALT and elevated ALT were(18324.61±4090.64),(20277.33±3315.42)ng/ml,respectively.The serum sVAP-1 level in CHB patients with elevated ALT level was higher than that in CHB patients with normal ALT level,the difference are statistically significant(t=2.10,P<0.05).(7)The serum HA levels in CHB patients with normal ALT and elevated ALT were(3525.92±562.76),(3873.68±239.52)pg/ml,respectively.The serum HA level in CHB patients with elevated ALT level was significantly higher than that in CHB patients with normal ALT level,the difference was statistically significant(t=3.22,P<0.005).(8)The correlation analysis of serum sVAP-1 level with serum HA level and liver function in CHB patients:sVAP-1 level was positively correlated with HA level,the correlation coefficient r=0.385,the determination coefficient R~2=0.148,P=0.030;sVAP-1 level was positively correlated with ALT level,the correlation coefficient r=0.074,the determination coefficient R~2=0.005,P=0.689;sVAP-1 level was positively correlated with AST level,the correlation coefficient r=0.140,the determination coefficient R~2=0.020,P=0.446;sVAP-1 level was positively correlated with Tbil level,the correlation coefficient r=0.062,the determination coefficient R~2=0.004,P=0.735;sVAP-1 level was positively correlated with gamma-GT level with correlation coefficient r=0.116,determination coefficient R~2=0.013,P=0.526;sVAP-1 level was negatively correlated with Alb level,the correlation coefficient r=-0.234,the determination coefficient R~2=0.055,P=0.197.Conclusion(1)sVAP-1 level may be related to the development of liver fibrosis in patients with chronic hepatitis B.(2)The level of sVAP-1 may be related to the level of hepatic inflammation in patients with chronic hepatitis B.(3)sVAP-1 level may be associated with HBV DNA replication in patients with chronic hepatitis B.