Expression And Significance Of Estrogen Receptor And Progesterone Receptor In Non-small Cell Lung

Objective:To detect the expression of estrogen receptor alpha(ER?)and progesterone receptor(PR)in non-small cell lung cancer,and compare their expression in benign lung tumors and adjacent normal lung tissues;To analyze the relationship between the expression of ER? and PR in non-small cell lung cancer tissues and the clinicopathological features and prognosis of patients,and to evaluate whether ER? and PR can be used as indicators to predict the prognosis of patients with non-small cell lung cancer.Methods:1.To retrieve the patients who were diagnosed as non-small cell lung cancer by surgical resection and pathological diagnosis from 2011 to 2016 in the information library of the First Affiliated Hospital of Wannan Medical College.Collect complete clinical information about the patient,including age,gender,female menopause history,smoking history,primary mass size,and lymph node metastasis.Screening of effective cases,355 cases including 180 cases of lung adenocarcinoma,lung squamous cell carcinoma of 175 cases.At the same time,20 cases of benign lung tumor tissues and adjacent normal lung tissues(>5.0cm from tumor)were randomly selected as control group.2.Selecting effective tissue wax blocks after screening and processed into tissue microarrays by tissue microarray technique,and the expression of ER? and PR was detected by immunohistochemical staining.Due to the phenomenon of detachment of tissue microarray,after secondary screening,there were 331 effective cases of NSCLC,18 cases of benign lung tumors,and 20 cases of adjacent normal lung tissues.To compare the expression of ER? and PR in non-small cell lung cancer tissues,benign lung tumor tissues and adjacent normal lung tissues;to analyze the correlation between the expression of ER? and PR and its correlation with clinical features and prognosis of patients;to explore the relationship between survival prognosis and clinical features in patients with non-small cell lung cancer.3.Using the statistical software for statistical analysis.Constituent ratio difference use chi-square test,at the same time Kaplan-Meier survival analysis curve was used to study the relationship between clinical features and prognosis of patients with non-small cell lung cancer.Results: Among the 331 patients with non-small cell lung cancer,there were 248 male patients and 83 female patients.The age ranged from 26 to 84 years old,with a median age of 62 years and an average survival time of 32.69 months.The positive expression rates of ER? and PR in non-small cell lung cancer tissues were 3.32%(11/331)and2.11%(7/331),respectively.The positive expression rates in lung benign tumor tissues were 27.78%(5/18),22.22%(4/18),and ER? and PR were negatively expressed in normal lung tissues adjacent to the cancer.The expression of ER? and PR in NSCLC tissues was not statistically different from the gender,age,female menopause history,smoking history,primary tumor size,degree of differentiation,histological type,lymphatic metastasis and clinical TNM scores(P > 0.05).There was no significant difference in the positive expression of ER? and PR in NSCLC;the positive expression of ER? and PR in NSCLC was not related to the prognosis of patients.The survival prognosis of patients with non-small cell lung cancer was related to the degree of pathological differentiation,lymph node metastasis and clinical TNM stage(P<0.05),but there was no correlation with gender,age,female menopause history,smoking history and histopathological type(P>0.05).Conclusion:1.ER? and PR were expressed in NSCLC and benign lung tumors,and no expression was found in normal lung tissues.It suggests that there may be a certain correlation between the occurrence and development of estrogen and progesterone;2.There is no correlation between ER?,PR and clinical features and prognosis of patients with NSCLC.It is not yet an indicator for predicting the prognosis of patients with NSCLC.