Study On Knockdown Of PBX1 To Improve Radiosensitivity Of Oesophageal Squamous Cell Carcinoma And Its Mechanism

Background:The radioresistance of oesophageal squamous cell carcinoma(OSCC)is a critical factor leading to a poor prognosis among patients.The expression of PBX1 is abnormally high in a broad range of human tissues,and this gene plays a key role in tumour proliferation.This research intended to explore the radiosensitization of OSCC by silencing PBX1.Methods:The OSCC cell lines KYSE450 and KYSE150 were subjected to PBX1 silencing and/or irradiation(IR).Cell proliferation,colony formation,and apoptosis were tested to evaluate the radiosensitization ability of PBX1 silencing.The levels of STAT3 and p-STAT3 in the OSCC cells were tested by Western blotting.Furthermore,KYSE150 cells with or without PBX1 silencing were xenografted into nude mice with or without radiation exposure.Results:Concomitant PBX1 silencing and IR can obviously suppress growth and enhance radiosensitivity in OSCC cells and xenografts.Moreover,the downregulation of PBX1 inhibits the expression of STATS and p-STAT3.Conclusions:The downregulation of PBX1 may increase radiosensitivity in OSCC cells and xenografts via the PBX1/STAT3 pathway.Our findings demonstrate that PBX1 may be a potential target for promoting the effect of radiation therapy in OSCC patients.