Effect Of Oleic Acid On Lipopolysaccharide-induced Inflammatory Response In Mouse Macrophages And Its Mechanism

Objective: To investigate the effect of oleic acid on lipopolysaccharide-induced inflammation and proliferation of mouse macrophages,and to explore its mechanism.It provides new targets and ideas for clinical prevention and treatment of T2 DM and metabolic syndrome.Methods1.Real-time PCR was used to determine the mRNA level of IL1β,IL6,COX2,iNOS and TNF-α in mouse macrophage inflammatory cytokines induced by LPS,as well as the corresponding dose-response and aging relationships,and to verify and construct the inflammatory response model.And on this basis to explore the effects of oleic acid on lipopolysaccharide-induced inflammatory cytokines and NF-κB signaling pathway.2.The effect of oleic acid on the proliferation of mouse macrophages induced by lipopolysaccharide was assessed by EdU proliferation assay.3.The NF-κB and pNF-κB P65 expression level were measured by Western blotting to explore the mechanism of oleic acid on inflammatory response.Result:1.Real-time PCR showed that the inflammatory response of macrophages in mice induced by LPS at 1mg/mL for 12 h was the most effective.Oleic acid inhibited the mRNA transcription of IL1β,IL6,COX2,iNOS,TNFα and signaling factor NF-κB induced by LPS(P<0.05).2.EdU proliferation test results showed that oleic acid inhibited the proliferation of mouse macrophages induced by LPS,while BSA group showed no inhibitory effect.3.WB results showed that oleic acid down-regulated the expression of NF-κB and pNF-κB P65 proteins in mouse macrophages induced by LPS,while BSA group had no significant effect.Conclusions:1.Oleic acid inhibited the lipopolysaccharide-mediated inflammatory response of mouse macrophages.2.The inhibitory effect of oleic acid on lipopolysaccharide induced inflammation in mouse macrophages may be related to the downregulation of NF-κB expression.