The Expression Profile And Functional Analysis Of Circular RNAs In Gastric Cancer

Objectives:To screen and identify the expression profile of circular RNA in gastric cancer cells and analyze its biological function.Methods:Human poorly differentiated gastric cancer cells MGC-803,human moderately differentiated gastric cancer cells SGC-7901,human highly differentiated gastric cancer cells NCI-N87 and human normal gastric epithelial cells GES-1 are cultured in vitro.The differentially expressed circRNAs in gastric cancer cells and normal gastric epithelial cells were detected by circRNA gene chip technology.Some differentially expressed circRNAs are validated by qRT-PCR.Cytoscape software is used to construct circRNA-microRNA-RNA network map,GO analysis and KEGG Pathway analysis are performed on downstream target genes.Results:Compared with normal gastric epithelial cells,79 up-regulated circRNAs and 229 down-regulated circRNAs were expressed in human gastric cancer cells with different differentiation degrees.The results of PCR indicated that hsa_circRNA₁03571 was significantly higher in normal cells than in cancer cells.GO analysis indicated that the target genes indirectly regulated by hsa_circRNA₁03571 mainly focused on binding proteins andregulating the activity of protein kinase,cell components mainly concentrated on organelles,biological processes mainly involved in protein modification.KEGG Pathway analysis suggests that hsa_circRNA₁03571 may participate in the development of gastric cancer through Ras signaling pathway,FoxO signaling pathway,Hippo signaling pathway and mTOR signaling pathway.Conclusions:In gastric cancer cells,hsa_circRNA₁03571 is a tumor suppressor gene,and its mechanism may be to interact with hsa-miR-16-5p,and participate in tumor development through multiple signaling pathways such as Ras signaling pathway and Hippo signaling pathway.