Study On The Mechanisms Of Astragalus Angelica Compatibility Of Cancer Cachexia

ObjectiveStudy on the effect of Astragalus Angelica compatibility on cancer cachexia and its optimal compatibility ratio,and to explore the mechanism.To provide modern experimental data support for the theory of TCM treatment of cancer cachexia,and to provide new ideas for diagnosis and treatment.Method1.Effect of Astragalus Angelica compatibility on cancer cachexia:48 mice were divided into blank group,model group,positive group,5:1 group,1:1 group and 1:5 group.The model was established(CT-26 cells were planted under the right axilla of mice,the general situation,food intake and weight loss rate of mice were observed).After evaluating the model,the gastrocnemius muscle of mice was taken after 12 days of drug intervention,and muscle tissue was observed.The contents of blood sugar,albumin,total protein,triglyceride,TNF-_and IL-6 were detected.2.Mechanisms of Astragalus Angelica compatibility intervention on cancer cachexia:Mouse gastrocnemius muscle tissue was taken,IRS-1 PI3K expression in gastrocnemius muscle tissue was detected by Real-Time PCR,and AKT mTOR expression in gastrocnemius muscle tissue was detected by Western Blot and HIC.Result1.Effect of Astragalus Angelica compatibility on cancer cachexia:After model maturation,each group was intervened with corresponding drugs for 12 days.The weight of mice in model group and positive group was significantly lower than that in blank group(P<0.05).There was no difference in weight between Astragalus Angelica compatibility groups and blank group(P>0.05).The wet weight of gastrocnemius muscle in each group was significantly different from that in the blank group(P<0.05).The wet weight of Astragalus Angelica 5:1 group was significantly heavier than that in the positive group(P<0.01).The average tumor weight of mice was:model group>Astragalus Angelica 1:1 group>Astragalus Angelica 1:5 group>positive group>Astragalus Angelica 5:1 group,but there was no difference among the groups.Biochemical indicators showed that the contents of glucose and total protein in the positive group and the compatible group of Astragalus and Angelica were higher than those in the model group,especially in the compatible group of Astragalus and Angelica 5:1 and 1:5,the difference was significant(P<0.01).Muscle tissue injury degree:model group>Astragalus Angelica 1:5 group>Astragalus Angelica 1:1 group>positive group>Astragalus Angelica 5:1 group>blank group.Cross-sectional area and diameter of muscle fibers:Compared with the blank group,the cross-sectional area and diameter of muscle fibers in model group and positive group were significantly reduced.The combination of Astragalus Angelica 5:1 and 1:1 could repair the muscle fibers in varying degrees,while the combination of Astragalus Angelica 1:5 could aggravate the injury of muscle fibers.2.Mechanisms of Astragalus Angelica compatibility in intervention of cancer cachexia:TNF-a and IL-6 in model group were significantly higher than those in blank group,with significant difference(P<0.01);the expression of both was significantly decreased by the compatibility of Astragalus and angelica,P<0.01.The expression of IRS-1 and PI3K was low in the model group,and the upstream factors were up-regulated in the compatibility groups of Radix Astragali and Angelica Sinensis,especially in the 5:1 group of Radix Astragali and Angelica Sinensis(P<0.01).The expression of AKT and mTOR in model group was significantly lower than that in blank group(P<0.01).The expression of AKT and mTOR in Astragalus Angelica group was significantly higher than that in blank group(P<0.01),especially in Astragalus Angelica 5:1 group.Conclusion1.Astragalus Angelica compatibility can significantly improve the general condition of mice,increase food intake,alleviate weight loss and muscle atrophy.Astragalus Angelica 5:1 compatibility has the best effect.2.The mechanism of Astragalus Angelica compatibility improving cancer cachexia may be through interfering with IGEF-1/PI3K/AKT/MTOR signaling pathway.Specifically,it can up-regulate IGF-1,activate downstream PI3K and AKT,promote the expression of mTOR and increase myosin synthesis.