Propofol Inhibits Invasion And Migration Of Breast Cancer Cells Via Down-regulating Transforming Growth Factor-?1

Background:Breast cancer is the most common malignant tumor in women,and the main treatment is surgery.The influencing factors of tumor survival rate include tumor marker level,clinical stage,pathological grade,residual lesion size,lymph node metastasis and clearance.In addition,the influence of anesthesia on tumor cell growth,recurrence and metastasis has gradually become one of the hot topics of research.As a star drug of general anesthesia,this study explored the effect of propofol on tumor cells,and provides a new theoretical basis for the selection of appropriate anesthesia programs in the future.Objective:To investigate the effect of propofol on the migration and invasion of breast cancer cells by regulating TGF-?1.Methods:The effect of different concentrations of propofol on the proliferation of breast cancer cells was detected by CCK-8 method.theexpression of TGF-?1,MMP-9,MMP-2 were detected by westernblot,Transwell migration assays,Matrigel invasion assays,and vascular mimicry assys were performed to assess cell migration,invasion,and vascular mimicry formation in propofol treatment or overexpression of TGF-?1 treatment.Results : Propofol inhibited the proliferation of MCF-7 cells,and down-regulating the expression of TGF-?1,MMP-9 and MMP-2 in MCF-7cells.Functional tests showed that propofol inhibited tumor cell migration,invasion and vascular mimicry formation in vitro.Overexpression of TGF-?1 reverses the inhibitory effect of propofol on breast cancer cells.Conclusion:Propofol can inhibit the migration and invasion of breast cancer cells in vitro by down-regulating TGF-?1.