| ObjectiveGingival recession is a common clinical problem and surgical root coverage is an effective method to treat gingival recession.Gingival recession associated with carious or non-carious dental cervical lesions is very difficult in clinical treatment.It has become a research hotspot to find a biocompatible material to increase the bonding ability between the material and gingival tissue and improve the effect of root coverage which was performed on the restored root surface.The objective of this study was to explore the possibility of gingival tissue adhering to root surface that was restored with chitosan modifying glass ionomer cement in the case of gingival recession associated with root lesion,which provided a theoretical basis for clinical application at the cellular level.MethodsThe cylindrical specimens were mixed by hand according to the manufacturer's instructions after integrating 1wt%,2wt%,4wt%chitosan?CS,30KDa,DD=90%?into glass ionomer cement?GIC?fluid.Fourier transform infrared spectrometry analysis?FTIR?was carried out to determine the success of preparing the chitosan modifying glass ionomer cement?CS-GIC?and scanning electron microscopy?SEM?was carried out to observe the surface microstructure.Mechanical properties?compressive strength,diametral tensile strength?were measured by Universal Testing Machine up to 5 days.Fractography of these specimens were analyzed by SEM.Cytotoxicity was assessed by CCK-8 assay using the extracts from materials,after detecting pH value of the extracts.Adhesion and proliferation of human gingival fibroblasts?HGFs?on the surface of materials were observed,using calcein-AM and SEM respectively.Alamar Blue assay was performed to quantitatively evaluate cell proliferation on material surface.ResultsFTIR showed that CS modifying GIC had a new characteristic peak at 1716 cm-1.Besides,2wt%CS-GIC had better performance than other test groups.The surface of2wt%CS-GIC was observed less microcracks under SEM.2wt%CS-GIC could enhance the compressive strength of original GIC at 5 days?P<0.05?and the addition of chitosan didn't change the basic fracture mode of materials.Additionally,the integration 2 wt%CS into GIC can obviously reduce acidity of the original GIC?P<0.01?when using extracts with concentrations of 100 and 50%.The Cell Counting Kit-8 assay and adhesion and proliferation of human gingival fibroblasts?HGFs?on the surface of the materials indicated that 2 wt%CS-GIC presented better cytocompatibility and was more suitable for the growth of HGFs.In contrast,1wt%CS-GIC or 4wt%CS-GIC did not enhanced the mechanical properties of original GIC,did not reduced the acidity of the extracts and did not promoted the proliferation of HGFs significantly.ConclusionThe results of this study showed that,CS could be successfully integrated into GIC which affected the characteristics and cytocompatibilities of GIC.2wt%CS-GIC could effectively improve the mechanical properties and cytocompatibility of original GIC.It can be considered as root filling material to promote the adhesion and growth of gingival tissue after performing root coverage on the surface of materials.In addition,this experiment laid the foundation for in vivo experiments,thus providing a new way to solve clinical problems. |
PDF Full Text Request