Effect Of Sanhuang Yishen Capsule On The Expression Of NF-?B And MCP-1 In Kidney Tissue Of Early Diabetic Nephropathy Rats

Objective: To observe the effect of Sanhuang Yishen Capsule on the expression of nuclear factor-kappa B(NF-?B)and monocyte chemoattractant protein-1(MCP-1)in kidney tissue of early diabetic nephropathy rats,and to explore the possible mechanism of Sanhuang Yishen Capsule in preventing and treating diabetic nephropathy.Methods: Seventy male Sprague-Dawley rats were randomly divided into normal control group(NC)and model group(60 rats)after one week of adaptive feeding.After intraperitoneal injection of streptozotocin(STZ),the model group was randomly divided into diabetic nephropathy group(DN),ramipril group(RP)and Sanhuang Yishen capsule low(SL),medium(SM)and high(SH)dose groups,with 10 rats in each group.Rats in SL group,SM group and SH group were given Sanhuang Yishen Capsule 0.4 g/kg/d,0.8g/kg/d and 1.6 g/kg/d respectively.Rats in RP group were given ramipril tablet0.5 mg/kg/d,and rats in NC group and DN group were given equal dose of distilled water.At the end of the 8th week,the blood glucose,triglyceride,cholesterol,blood urea nitrogen,serum creatinine,24-hour urinary microalbumin,renal pathological changes and the expression of NF-?B and MCP-1 in renal tissues were observed.Results:1.General conditions of rats in each groupRats in NC group were in good mental state,with normal eating and activities.The mental state of rats in DN group was poor,and the symptoms of rats in the treatment groups were improved compared with those in DN group.2.Changes of blood glucose,triglycerides,cholesterol,blood urea nitrogen,serum creatinine and 24-hour urinary microalbumin of rats in eachgroupAfter treatment,blood glucose,triglycerides,and cholesterol of rats in each group were not significantly improved.The blood urea nitrogen,serum creatinine,and 24-hour urinary microalbumin of rats in each group were lower than those in DN group,while those in SM group,SH group and RP group were significantly reduced.3.Pathological changes of kidney tissue in each groupThe renal tissue structure of rats in NC group was generally normal.The renal tissue structure of rats in DN group was disordered,the basement membrane was thickened,and inflammatory cells were infiltrated in the interstitial region.The pathological damage of renal tissue in each treatment group was alleviated to varying degrees compared with that in DN group,especially in SM group,SH group and RP group.4.Expression of NF-?B and MCP-1 in renal tissues of rats in each groupNF-?B,MCP-1 protein and MCP-1 mRNA were normally expressed in the renal tissues of rats in NC group,but were highly expressed in the renal tissues of rats in DN group,decreased in the renal tissues of the rats in each treatment group.The expression in the renal tissues of rats in SM group,SH group and RP group decreased significantly.Conclusions:1.Shanhuang Yishen Capsule can reduce serum creatinine,blood urea nitrogen,and 24-hour urinary microalbumin in early DN rats,alleviate and improve renal pathological damage in early DN rats.This therapeutic effect is independent of changes in blood glucose and blood lipids.2.Shanhuang Yishen Capsule can inhibit the expression of NF-?B,MCP-1 protein and gene in renal tissue.3.Sanhuang Yishen Capsule partly protect renal tissue and improve early DN in rats through the above mechanisms.