MicroPET-CT Demonstrates The Effect Of Vein Transplantation Of HUCB-NSCs In Intracerebral Hemorrhage Rat Model

Objective:To observe the functional recovery of rat models of cerebral hemorrhage before and after transplantation by microPET-CT and Longa scores,and to scientifically evaluate the transplantation of human cord blood-derived neural stem cells(HUCB-NSCs).The curative effect of treating cerebral hemorrhage in rats provides a scientific experimental basis for the treatment of cerebral hemorrhage by clinical cord blood stem cells.Method:A rat model of cerebral hemorrhage was established by two-step method.After successful modeling for 24 h,the rat model was randomly divided into transplantation group and control group.The transplantation group was re-anesthetized and fixed,and the micro-syringe transplantation was adjusted through the tail vein.A suspension of HUCB-NSCs with a cell density of 1 x 10~6/ml.After successful modeling in the control group,no treatment was given and the outcome was natural.The functional recovery of rat models of cerebral hemorrhage before,and after 3,7,14,and 21 days of transplantation of HUCB-MNCs was assessed by microPET-CT scan and Longa score.Results:1.Neurological function score in the 7,14 and 21 days after transplantation was significantly lower in the transplantation group than in the control group(P<0.05).Neurological function score in the control group decreased significantly from 14 days after transplantation.The transplant group nerve function score was started with 3 days after transplantation the declining trends,to 21 from heaven to normal.2.Glucose metabolism in hematoma center(SUV%)was significantly higher in the transplantation group than in the control group(P<0.05)at 3,7,14 and 21 days after transplantation.In the control group,SUV%in the hematoma center decreased 3 days after transplantation compared with that before transplantation,and increased significantly at 7,14 and 21 days after transplantation.However,SUV%of the hematoma center in the transplant group showed an increasing trend from 7 days after transplantation.3.Glucose metabolism around hematoma(SUV%)was significantly higher in the transplantation group than in the control group at 7,14 and 21 days after transplantation(P<0.05).SUV%around hematoma in the control group decreased at 3 days after transplantation compared with that before transplantation,and showed no significant change at 7,14 and 21 days after transplantation compared with that at 3 days after transplantation.However,SUV%in the transplantation group showed an increasing trend from 7 days after transplantation.4.The volume of cerebral hematoma and its surroundings in the rats at 7,14 and 21 days after transplantation was significantly smaller in the transplantation group than in the control group(P<0.05).However,the volume of hematoma and surrounding hematoma in the transplantation group showed a decreasing trend from the 7th day after transplantation.Conclusion:This study demonstrates that transplantation of human umbilical cord blood-derived neural stem cells can promote the recovery of neurological function in rats with cerebral hemorrhage.