The Repairing Effect Of Transplantation Of Bone Marrow Stromal Cells-derived Neural Stem Cells After Subarachnoid Hemorrhage In Adult Rats

Objective: Brain edema and serious neuron damage may take place through differentmechanisms, resulting from cerebral vasospasm and intracranial hypertension after aneurismalsubarachnoid hemorrhage(SAH),which lead to lack of neurological function of the patients, and theorthodox therapy is helpless for it. Neural stem cells transplantation has raised hopes thatresearchers can find ways to repair nervous system damage. Bone marrow stromal cells(BMSCs)can be the ideal source for transplantation due to their strong abilities to renew and differentiateand their abundance source without either ethical worries or potential immunology rejection.Theobjective of this pilot test is to study the repairing effect of transplantation of bone marrow stromalcells-derived neural stem cells(BMSCs-D-NSCs) on adult Wistar rats with SAH and provide moretheory support for neural stem cells transplantation.Methods: Bone marrow cells were collected from the femur and tibia of adult wistarrats. Then, BMSCs were harvested by means of density gradient centrifugation. BMSCs from adultwistar rats were cultured with neurabasal medium and EGF, bFGF. Immunocytochemistry wasperformed to identify the differentiated cells. BMSCs-D-NSCs were labeled with5-Bromo-2'-deoxyuridine(BrdU). 45 adult Wistar rats were randomly divided into the threegroups of control,DMEM(Dulbecco's Modified Eagle Medium) and BMSCs-D-NSCs after beingsuccessfully made into the models of SAH. The SAH models were induced by injection of 0.3mlautologous blood into the cisterna magna twice, 24 hours later, the DMEM group andBMSCs-D-NSCs group were respectively induced by injection of 30ul DMEM and 30ul cell(3-6×10~6BMSCs-D-NSCs) suspension into the cisterna magna of the SAH models.The neurological functional grade score was used to evaluate the neural function of rats atdifferent intervals following the transplantation. The BrdU positive cells were detected withimmunohistochemical staining method.Results: At 5d after SAH, there was no statistical difference in neurological functional gradescore of the three groups(F=0.075,P＞0.05).At 10d,20d,30d and 40d after SAH, the differencebetween the SAH group and the DMEM group was insignificant(P＞0.05), but compared witheither of other groups, BMSCs-D-NSCs group was observed amelioration in the neuralfunction (P＜0.05); The BrdU positive cells were detected in the brains of the rats.Conclusion: Transplantation of BMSCs-D-NSCs can effectually improve the neuralfunction of rats with SAH; The new generated neurons differentiated fromBMSCs-D-NSCs may exert a repairing influence on the brain after SAH.