Scale Preparation Of Sol-gel Bioactive Glass And The Study Of Porous Bone Repair Scaffold

Bone defects caused by trauma and tumor surgery are common clinical diseases,and the synthetic biomedical materials provide new ideas for the treatment of bone defects.As a kind of inorganic non-metallic material with good biological activity,bioactive glass can form a strong chemical bond with bone tissue and promote new bone formation.The sol-gel bioactive glass is highly concerned with biomedical materials and clinical treatments due to its large specific surface area and uniform chemical composition.However,in the past,sol-gel bioactive glass was mostly prepared in small samples in the laboratory with a small yield of tens of grams.How to achieve large-scale and stable production is a key technical bottleneck restricting the commercial conversion of such materials.Based on this,this paper analyzes the related issues of the scale preparation of bioactive glass.Through the assembly design of the device model and the optimization of the reaction process,the large-scale preparation of sol-gel bioactive glass with a single production of 5kg was realized,which laid a material foundation for further research and development of bioactive glass tissue repair products.In addition,the bioactive glass bone repair products currently used in clinical applications are mostly powder granules,which is not conducive to the filling of larger size bone defects and it is easy to be flushed out by blood.Therefore,in this thesis,a bioactive glass porous bone repair scaffold was prepared by using the sol-gel bioactive glass powder obtained by large-scale preparation as a raw material with the pore-forming agent and a compression molding process.The main research work and conclusions are as follows:(1)The sol-gel bioactive glass was prepared,and its reaction mechanism and physical and chemical properties were analyzed and characterized.It has a specific surface area as high as 187.80m~2g~-11 and an average pore diameter of 5.39nm,which confirms its good biological activity and provides a theoretical foundation for subsequent large-scale production process.(2)The large-scale preparation of sol-gel bioactive glass has be achieved.Through the independent design of the experimental model,the linkage use of the solid-liquid material adding device is realized,and the process of the sol-gel reaction system is optimized by"uniformly inputting materials","stable hydrolysis reaction",and"dispersedly aging into glue".Consequently the reaction concentration and the aging temperature gradient are stably controlled,and the fine uniformity under a large reaction space is solved.A series of physicochemical properties,in vitro mineralization and cytological properties of the powders prepared by scale were studied.The study found that the material composition is uniform,with mesoporous structure,high specific surface area and excellent mineralization,and has excellent biocompatibility.All physical and chemical properties are stable and reproducible.It can promote the proliferation of mBMSCs cells with in a certain concentration.This material can be used as a quality raw material for bioactive glass related products.(3)The bioactive glass porous bone repair scaffold was prepared by using the sol-gel bioactive glass prepared by large-scale preparation as raw material,mixed with porogen,combined with compression molding and cold isostatic pressing.The effects of different porogens on the heat treatment process,morphology and porosity of porous bone prosthesis were preliminarily investigated.The in vitro degradation process and mechanical properties of the material were tested,and the evaluation of cell biology and in vivo osteogenic performance was carried out.The study found that,the porosity of the porous bone repair scaffold prepared by using mannitol as pore-forming agent can be adjusted between 40%and60%,with a suitable and gradient distribution pore size,pore size of about 50-150μm,and micropores of 5μm-20μm.The compressive strength is about 5Mpa and it has good biocompatibility,which is conducive to the adhesion and proliferation of osteoblasts.The osteogenesis of is good which has potential for clinical application,a large amount of new bone tissue is formed within 2 months,and the implant is tightly wrapped.In summary,we successfully completed the large-scale preparation of sol-gel bioactive glass,and through a series of test characterization proved that the bioactive glass has good physical and chemical properties and biocompatibility.As a raw material,a bioactive glass porous bone repair scaffold was prepared.This work provides high-quality raw materials and theoretical basis for the further development of bioactive glass related products.