CPC/CMC-CD55sp Nanoparticles Inhibit Proliferation And Apoptosis Of Caski Cells In Vitro

Objective:To construct phycocyanin/carboxymethyl chitosan-CD55 specific ligand peptide(CPC/CMC-CD55sp)nanoparticles,and to study the anti-cancer effect and mechanism of CPC/CMC-CD55 sp nanosphere on cervical cancer(Caski)cells.Methods:The surface of nanoparticles was ionically crosslinked with CD55 specific ligand peptide(CD55sp)to form CPC/CMC-CD55 sp nanoparticles,and CMC was used as carrier to encapsulate CPC to construct nanoparticles.Targeted delivery of CD55 ligand peptide was accomplished by its specific binding with CD55 on the surface of Caski cells.The experiment was divided into four groups: Control group,CPC group,CPC/CMC group(non-target group),CPC/CMC-CD55 sp group(target group).The drug safety was tested by hemolysis test;CCK-8 test and cell cloning assay to detect the inhibitory effect of on Caski cells;the expression of CD55,the uptake rate of phycocyanin and cell apoptosis were detected by flow cytometry;and scratch test and Transwell migration test to detect the effects of drugs on cell migration;the expression of cell surface proteins CD55,apoptosis-related proteins(cleaved caspase-3 and Bcl-2)and cycle-related proteins(AKT,CyclinD1,CyclinE1,CDK4 and P21)were detected by Western Blot;the uptake of phycocyanin by Caski cells was observed by fluorescence microscopy.Result:The results showed that Caski cells was highly expressed complement regulatory protein CD55.And in the guidance of CD55 sp,nanoparticles can reach the surface of Caski cells targeting and efficiently,so the uptake rate of phycocyanin in targeted group is much higher than that in non-targeted group and phycocyanin group.Tts drug safety is good,and less side effects.Nanoparticles can significantly inhibit the proliferation and migration and can induce apoptosis of Caski cells.Further studies showed that CPC/CMC-CD55 sp nanoparticles could up-regulate the expression of P21 protein,down-regulate the expression of AKT,CyclinD1,CyclinE1 and CDK4 protein,thus inhibiting the cell cycle and inhibiting the proliferation of Caski cells.And up-regulating the expression of cleaved caspase-3 protein and down-regulating the expression of Bcl-2 protein to induce apoptosis of Caski cells.Conclusion:CPC/CMC-CD55 sp nanoparticles can target the surface of cancer cells,inhibit the migration and proliferation of cervical cancer Caski cells,and induce apoptosis.It improves the stability and utilization rate of phycocyanin as an antineoplastic drug,and opens a way for the development of antineoplastic marine drugs.