The Research Of The Effect Of Resveratrol On Benign Prostatic Hyperplasia Epithelial Cells

Objective Our present research aims at exploring the probable mechanism of apoptosis effect of resveratrol on BPH by using Benign Prostatic Hyperplasia Epithelial Cell Line(BPH-1).Methods PART1: BPH-1 cells were divided into 2 groups,named as control group and resveratrol group,various concentration of resveratrol(Res)was used to treat on BPH-1,MTT assay was used to investigat the cells viability and to confirm the concentration and time,apoptosis,ROS level were assessed after administration,then activation of p38 MAPK was detected,and protein level of FOXO3 a,Bcl2,Bcl-XL,caspase3 was measured by western blot.PART2: cells were divided into control group,resveratrol group,Res+NAC group and Res+SB group,those groups were treated with DMSO,30?mol/L Res,30?mol/L Res+5mmol/L NAC,30?mol/L Res+5?mol/L SB,MTT assay was used to measure the cells viability,ROS level were assessed by Flow cytometry,apoptosis associated proteins and the level of p38 MAPK,Foxo3a,SOD2,Catalase were measured by western blot.Results 1.cells vablity was decreased after treatment,and this effect was time and dose dependented,the IC50 of 24 h and 48 h were 22.91?mol/L,11.21?mol/L.2.the apoptosis rate after administration were control group(0.24±0.19)%,20?mol/L Res(2.57±0.53)%,30?mol/L Res(4.32±1.07)%,and the ROS level was elevated with the dose increasing.3.resveratrol promoted the phosphorylation of p38 MAPK,and downregulated the expression of Foxo3 a,Bcl2 and Bcl-XL,increased the cleaved-Caspase3.4.NAC and SB repressed the proliferate inhibition,apoptosis and ROS level induced by resveratrol.5.compared with Res group,the phosphorylation of p38 MAPK was decreased,the level of Foxo3 a,SOD2,Catalse,Bcl2,Bcl-XL were elevated,cleaved-Caspase3 was reduced.Conclusions 1.Resveratrol can induce the apoptosis of BPH-1,and this effect may be associated with the ROS accumulation.2.resveratrol lead to the downregulation of Foxo3 a,and the level of SOD2,Catalase were decreased,thus,casuing the decrease of the ability of ROS clearing,as a result,the ROS level was elevated.3.resveratrol induced the increase of the phosphorylation of p38 MAPK,leading to the elevation of p-Foxo3 a,enhancing the Ubiquitination degradation of Foxo3 a,this may be the probable reason for the decreas of Foxo3 a.4.there may exist some positive feedback beteween the ROS level and p38 MAPK,ROS promote the phosphorylation of p38 MAPK,and the phosphorylation of p38 MAPK may promote the accumulation of ROS.