Study On Association Of Interleukin-36 And Interleukin-36 Receptor Antagonist Inflammatory Cytokine With Streptococci Infection And Geographic Tongue

Background: The etiology of geographic tongue is still unclear.Geographic tongue also called benign migratory glossitis(BMG)which could appear in malnutrition,atopic dermatitis,connective tissue diseases,and psoriasis and so on,in particularly generalized pustular psoriasis.Object: To sought the relationship between Group A streptococci infection and geographic tongue.Method: Collected sample from the outpatients with scarlet fever attending the Dermatology department in Xinhua Hospital of Shanghai Jiao Tong University.To compare prevalence of geographic tongue in scarlet fever and controls.Comprehensive sequencing of the IL36 RN gene was performed for the scarlet fever patients who grouped according to clinical features with or without geographic tongue.ELISA were performed to detect the expression of IL36 s,IL36Ra and CCL20 at protein level in scarlet fever patients with or without geographic tongue and controls.Result: There were 17 of 79 scarlet fever patients got geographic tongue,and 4 of 108 controls got geographic tongue.There were 3 of 79 scarlet fever patients had IL36 RN gene mutations,and 7 of 108 controls had IL36 RN gene mutations.Serum levels of IL-36s(IL-36?,IL-36?,and IL-36?)and IL-36 Ra were significantly increased in Scarlet fever(n=13)compared with those of healthy controls(n=8)(P<0.01).In patients,compared with geographic tongue group(n=7)and non-geographic tongue group(n=6),serum levels of IL-36 s and IL-36 Ra were significantly increased in geographic tongue group(P<0.05).In controls,compared with geographic tongue group(n=4)and non-geographic tongue group(n=4)was no significant(P> 0.05).In geographic group include patients with scarlet fever(n=7)and healthy(n=4),serum levels of IL-36 s and IL-36 Ra were significantly increased in patients with scarlet fever(P<0.01).In non-geographic tongue group include patients with scarlet fever(n=6)and healthy(n=4),serum levels of IL-36?,IL-36? and IL-36 Ra were no significant(P> 0.05),however IL-36? was significantly increased(P<0.01).Also comparing patients with non-geographic tongue(n=6)and healthy with geographic tongue(n=4),serum levels of IL-36? was significantly increased(P<0.01).Serum levels of IL-36?,IL-36? and IL-36 Ra were no significant(P> 0.05).Salivary IL-36??? and IL-36 RN m RNA were significantly increased in Scarlet fever(n=6)compared with those of healthy controls(n=6)(P<0.01).In patients,compared with geographic tongue group(n=3)and non-geographic tongue group(n=3),salivary IL-36? and ? m RNA were significantly increased in geographic tongue group(P<0.01).In controls,compared with geographic tongue group(n=3)and non-geographic tongue group(n=3),salivary IL-36??? and IL-36 RN m RNA was significant(P< 0.05).Conclusion: Our results demonstrated that infection could promote the secretion of inflammatory cytokine IL-36 s,while their antagonist IL-36 Ra could be released to disrupt the inflammatory circle,especially in patients with geographic tongue.It suggested that healthy people with geographic tongue could also secrete a lot of IL36 s and IL36 Ra in serum with the occurrence of infection.It could speculated that infection release large quantities of inflammatory cytokines IL36 s while IL36 Ra was not enough to antagonize inflammatory response,which exacerbated the march of generalized pustular psoriasis.