The Expression And Significance Of Tenascin-C In Prostate Cancer

Objective:As an extracellular matrix,Tenascin-C is involved in the proliferation,migration and invasion of various tumors.The expression of TN-C protein in prostate cancer tissues was examined,and the clinical pathological significance of TN-C was analyzed.Methods:Collecting wax blocks from 57 patients diagnosed as prostate cancer from 2010 to 2018 in the Affiliated Hospital of QingHai University.All patients had complete clinical data,including age,ethnicity,grade and grouping of Gleason,clinical stage,lymph node metastasis,bone metastasis,radiotherapy and chemotherapy,etc.The expression of TN-C in 57 PCa tissue samples was detected by Immunohistochemistry.To analyze whether the expression of TN-C was statistically significant with the clinical indicators of prostate cancer.Further analysis the relationship between the expression of TN-C in prostate cancer patients and the expression of Ki67,MMP2 and cancer associated fibroblasts(CAFs)related factors(SMA,Vimentin and FSP1)in prostate cancer.To explore the clinicopathological significance of TN-C expression in prostate cancer tissues.Statistical analysis of the data was performed using IBM SPSS 23.0 statistical software.Results:1.Among the 57 patients with prostate cancer,statistical analysis showed that TN-C expression in prostate cancer stroma was no significantly associated with age and ethnic distribution(P>0.05).2.There was statistical significance between the expression of TN-C in PCa tissues and Gleason grading group(P < 0.05),and the higher the Gleason grading group,the higher the positive rate of TN-C expression.Gamma(P)=0.607(P<0.001).There was statistical significance between the expression of TN-C in PCa tissues and clinical stage(P < 0.05),and the higher the clinical stage,the higher the positive rate of TN-C expression.Gamma(P)=0.609(P<0.001).3.The positive rate of TN-C in the group with lymph node metastasis was 77.3%,and that of TN-C in the group without lymph node metastasis was 45.7%,showing a significant difference between the two groups(P < 0.05).The positive rate of TN-C in the bone metastasis group(92.9%)was significantly higher than that in the boneless metastasis group(46.5%).The difference between the two groups was statistically significant(P<0.05).4.The positive rate of TN-C in PCa patients with proliferation index <10% was 40.7%,and the positive rate in PCa patients with proliferation index ?10% was 73.3%.The difference between the two groups was statistically significant(P<0.05).5.Immunohistochemistry showed that TN-C and CAFs markers(SMA,Vim,FSP1)can be expressed in PCa cytoplasm of stromal fibroblasts.The positive rate of TN-C was 74.1% in the SMA positive group,43.3% in the SMA negative group,the difference between the two groups was statistically significant(P<0.05).The positive rate of TN-C was 78.3% in the Vim positive group,and 44.1% in the Vim negative group,the difference between the two groups was statistically significant(P<0.05).The positive rate of TN-C in the FSP1 positive group was 71.0%,and the positive rate in the FSP1 negative group was 42.3%,the difference between the two groups was statistically significant(P<0.05).6.Immunohistochemistry showed that MMP2 was not only expressed in tumor cells,but also highly expressed in PCa stroma.In all the cases studied,the positive expression rate of TN-C in the MMP2 positive group(70.6%)was significantly higher than that of TN-C in the MMP2 negative group(39.1%),There were significant differences between the two groups(P < 0.05).Conclusion:TN-C expressed in PCa stromal fibroblasts,which may be a potential biomarker of CAFs and may represent high tumor invasion,suggesting poor prognosis of PCa and it may be one of the indicators to judge the poor prognosis of PCa.