Experiment Of GBR For Repair Of Peri-implant Alveolar Defects

PURPOSE:Guided bone regeneration(GBR)is usually applied to deal with the peri-implant alveolar bone defects.In the process,the choice of barrier membranes is crucial.This study aimed to evaluate the effect of using GBR with titanium mesh or Bio-Gide membrane or in combination in repair of alveolar bone defects in Beagle dogs.Thus provide reference for the treatment of peri-implant alveolar bone in clinical implant surgery.METHODS:The mandibular premolars and the first molar on both sides of the jaw in three dogs were extracted.Six months after extraction,three implants were inserted into each side,with alveolar bone defects(4mm×4mm×4mm)were produced.24 implants in total 6 sides were assigned to 3 groups and covered with different membranes,Group A:Implant+Bio-Oss+Ti-mesh,Group B:Implant+Bio-Oss+Bio-Gide,Group C:Implant+Bio-Oss+Ti-mesh+Bio-Gide.The claps were sutured over the membranes to complete the wound closure.The animals were sacrificed after 6 months,and non-decalcified histological specimens of the implants and surrounding tissues were prepared.Micro-CT scanning,fluorescence microscopy and Verhoeff-Van Gieson staining were carried out to test the results.SPSS17.0 software package was used for statistical analysis on the results and all values are presented as the mean±standard deviation(SD).One-way analysis of variance with Bonferroni test was performed to measure statistically significant differences between the three groups.A value of p<0.05 was considered significant.RESULTS:Micro-CT with three-dimensional reconstruction revealed that bone volume/total volume(BV/TV),trabecular number(Tb.N),trabecular thickness(Tb.Th)in Group C was significant better than the other two groups(P<0.01),while trabecular separation(Tb.Sp)in Group C was significant lower than the other two groups(P<0.01).In the fluorescence microscopy,the distance between the bands of three fluorescent dyes was significantly further in Group C(P<0.01),which indicates faster newly bone deposition.Verhoeff-Van Gieson staining shows that in Group C,the Bio-Oss particles got perfect integration with the newly deposited bone and the bone thickness was significant larger than the previous two groups.There was no significant difference between Group A and Group B.CONCLUSIONS:(1)The combination of Bio-Gide membrane and Titanium mesh may get a better bone regeneration effect than Titanium mesh or Bio-Gide alone.(2)This study suggests that combination of Bio-Gide membrane and Titanium mesh can effectively repair the peri-implant alveolar bone defects,so as to provide a new treatment idea and method in clinical implant surgery.