Study On The Expression Of VEGF And PCP-? In The Serum Of Neonates With Acute Respiratory Distress Syndrome

ObjectiveTo observe the expression of VEGF and PCP-? in the serum of children with acute respiratory distress syndrome(ARDS).Methods1.Selected newborns who received ventilator-assisted ventilation at the Neonatal Medical Center of Huai'an Maternal and Child Health Hospital,Huaian City,Jiangsu Province from July 2018 to June 2019.The enrolled newborns were categorized into ARDS and non-ARDS groups based on whether they were compliance with the diagnostic criteria for neonatal ARDS.2.According to the difference of oxygenation index(OI),the ARDS group was divided into three groups:mild group(4?OI<8);moderate group(8?OI<16);severe group(OI?16).3.The data of subject's sex,gestational age,birth weight,Downes score,number of invasive ventilation cases,time of oxygen therapy,P/F value,number of cases of PS application,and length of hospitalization.were collected.4.Serum VEGF and PCP-? expression levels were determined by enzyme-linked immuno sorbent assay(ELISA)at 24h,48h,and 7d postnatally in enrolled neonates.5.Statistical analysis of the data was performed by SPSS22.0 statistical package,and P<0.05 was considered statistically significant.Result1.A total of 118 newborns were selected and all of them were cured.There was no statistically significant difference between the two groups in gender,gestational age,birth weight,Downes score,premature rupture of membranes,neonatal asphyxia,high risk factors of pregnant mother and hospitalization time(P>0.05).The number of cases of invasive ventilation,the number of cases of PS application and the duration of oxygen therapy were significantly higher in ARDS group than those in non ARDS group(P<0.05).The P/F value of neonates in ARDS group was lower than that in non ARDS group,and the difference was statistically significant(P<0.05).2.There was statistically significant differences in serum VEGF level between ARDS group and non ARDS group at all time points after birth(P<0.05);serum VEGF levels were higher in the ARDS group than in the non-ARDS group at 24 hours postnatally,with a difference of statistically significant(P<0.05),the differences between the two groups at 48 hours postnatal and 7 days after birth were not statistically significant(P>0.05).3.Overall comparison of serum PCP-? levels between children in the ARDS and non-ARDS groups at all postnatal time points,with differences in the Statistically significant(P<0.05).There was no statistical significance in serum PCP-? levels between the two groups at 24 hours after birth(P>0.05),but higher in the ARDS group than in the non-ARDS group at 48 hours and 7 days after birth,The comparisons in both groups were statistically significant(P<0.05).4.At 24 hours postnatally,there was a significant difference in serum VEGF level between the three groups of ARDS children with mild,moderate,and severe ARDS(P<0.05).The level of VEGF in the moderate group and severe group was higher than that in the mild group(P<0.05).The level of VEGF in the mild group of ARDS children was the lowest,and that in the severe group was the highest;Statistically significant differences in VEGF levels between the three groups of children with mild,moderate,and severe ARDS were compared at 48 hours postnatally(P<0.05),The VEGF levels of children with ARDS in both the moderate and severe groups were higher than those in the mild group(P all<0.05),with the mild group Children with ARDS had the lowest levels and those in the severe group had the highest levels;,On the 7 days postnatally,compared with the VEGF levels of children with different degrees of ARDS,the VEGF levels of children with ARDS in the moderate group and the severe group were higher than those in the mild group,with the difference being statistically significant(p<0.05),in which children with ARDS were the lowest in the mild group and the highest in the moderate group.,5.The PCP-? level in children with mild,moderate and severe ARDS at 24 hours postnatally(19.48±5.40.27.50±10.56;40.90±10.85)pg/ml comparison,the difference was statistically significant(F=34.253,p=0.000),The PCP-7? level of children with moderate and severe ARDS weresignificantly higher than those in the mild ARDS(P<0.05).The PCP-? level of children with severe ARDS was significantly higher than those in the moderate ARDS(P<0.05);48 hours postnatal,the PCP-? level of children with mild,moderate and severe ARDS(22.41±8.30;28.17 ± 8.32;38.54 ± 8.94)pg/ml was statistically significant(F=20.281,P=0.000).The PCP-? level of children with moderate and severe ARDS was higher than that of children with mild ARDS(P<0.05).The PCP-? level of children with severe ARDS was higher than that of children with moderate ARDS(P<0.05)<0.05);On the 7 day postnatal,the PCP-? level of children with mild,moderate and severe ARDS(21.44 ± 7.64;28.59 ± 9.85;36.66 ± 7.73)pg/ml was statistically significant(F=18.455,P=0.000).The PCP-? level of children with moderate and severe ARDS was higher than that of children with mild ARDS(P<0.05).The PCP-? level of children with severe ARDS was higher than that of children with moderate ARDS(P<0.05)<0.05).Conclusion1.The serum VEGF level at 24 hours after birth,PCP-? level at 48 hours and 7 days after birth were significantly higher in ARDS group than in non ARDS group.2.Serum VEGF levels and PCP-? levels in children with ARDS are correlated with disease severity..