Expression Of FOXM1 In Chronic Periodontitis And Five Tycipal Endodontic Diseases Report

Objective: To study the expression of Forkhead box M1(FOXM1)in gingival tissue of chronic periodontitis,and to analyze the role of FOXM1 and provide some references for the clinical treatment of periodontitis.Method: Using Real-Time PCR and Western Blot to detect the expression of FOXM1 at the level of m RNA and gene in the clinical samples of chronic periodontitis.1.Clinical sample collection:(1)Collecting the gums of 15 patients with the moderate-severe periodontitis who underwent periodontal surgery as experimental group and the healthy gums of 15 patients with wisdom tooth flap extraction as control group during the period from 2018 to 2019 at Affiliated Stomatological Hospital of Da Lian Medical University.Before the operation,all patients were informed of the purpose of the experiment and signed the letter of consent.In addition,this experiment has also been approved by the Ethics Committee of xx University.(2)Clinic periodontal disease index:According to the IAP and the fourth edition of Periodontology to record gingival index(GI),pocket depth(PD),bleeding on probing(bleeding on probing,BOP)and clinical attachment loss(Clinical attachment loss,CAL)and other clinical indicators.Experimental group: 15 cases of gingival tissue with the moderate-severe periodontitis undergoing periodontal flap surgery,GI?2,PLI?2,PD> 5mm,BOP(+),CAL?3mm,bone resorption?1 / 3 root length.Control group: 15 cases of gingival tissues with third molar impacted tooth flap extraction,GI = 0,PLI=0,PD <3mm,BOP(-),CAL=0.2.Real-time PCR to determine the expression of FOXM1 m RNA:12 samples in the experimental group and the healthy control group were selected randomly for extracting Total RNA,reversing transcription to c DNA.Real-Time PCR was used to detect the expression of FOXM1 at the level of m RNA in gingival tissue.The images and date were used for statistical analysis.3.Western Blot to determine the expression of FOXM1 protein:Remaining 3 cases of periodontitis experimental group and 3 healthy control group were selected.After reactions such as total protein extraction,concentration determination,denaturation and electrophoresis,Western Blot was used to detect the expression of FOXM1 at the level of protein.The experimental strip Bands and data for statistical analysis.Results:1.Clinical sample collection results:15 patients with periodontitis were collected,including 8 males and 7 females,aged 23-59 years old;15 nomal control patients were 9 males and 6 females,aged 21-49 years old.2.Real-time PCR resultsThe results of Real-time PCR showed that FOXM1 m RNA was expressed in the gingival tissue of both control and experimental group.And the expression of FOXM1 at the level of m RNA in the gingival tissue of the experimental group was higher than that in the healthy control group,and the difference between the two groups was statistically significant(P <0.05).3.Western Blot resultsWestern Blot results showed that the FOXM1 protein was expressed in the gingival tissue of both control and experimental group.And the protein FOXM1 in the gingival tissues of chronic periodontitis was higher than that in the healthy control group.Thedifference between them was statistically significant(P <0.05).Conclusion: The expression of FOXM1 in gingival tissues of chronic periodontitis was high than the healthy gingival tissues at m RNA and protein levels,which revealed that FOXM1 played a role in the process of chronic periodontitis.