Curcumin Anti Inflammatory Response In Subarachnoid Hemorrhage And Resistance To Oxidative Stress

Hemorrhagic stroke,it is to point to a variety of reasons,such as hyperte-nsion,cere-bral aneurysm,and blood system diseases caused by bleeding in the brain parenchyma.Is more severe clinical cerebrovascular disease,accounting for 10%of stroke³0%,among them three months the case fatality rate of 20%to 30%.Is the most common clinical congenital intracranial aneurysm（AN）is broken,and accounts for about 50⁸5%,follo-wed by hardening of the arteries and blood vessel deformity,other such as arteritis,abn-ormal intracranial vascular disease,tumo,etc.Aneurysm rupture caused by subarachnoid hemorrhage is a serious cerebral hemorrhage,and deadly to a certain extent,but the sur-vivors can cause a long time of nerve function defect and cognitive impairment^[1].In the clinical treatment of subarachnoid hemorrhage mainly symptomatic treatment,and positive prevention and treatment of complications,such as:lower high blood pres-sure,reduce intracranial pressure and prevent the risk of bleeding again,etc.In the clinical treatment of focus is to reduce the mortality of the disease,reduce the morbidity of dise-ase,improve the cure rate of patients.Surgical treatment is mainly intervention,if haemo-rrhage amount is big for intracranial drainage,etc.Anti-inflammatory treatment of medical treatment,resisting oxidative stress treatment is a new direction.Humans first purification of curcumin is Japanese Hiroshi Hikino in 1966,China will in 1979 by Xu Hongxia curc-umin class material used in the treatment of cervical cancer,and obtained a certain curative effect.At present,the research field of curcumin is more and more widely,has been used to treat tumor in clinical,gastritis,hepatitis,etc.,in recent years,studies have used for the treatment of cerebral embolism.At present,the research of curcumin used in treating cere-bral hemorrhage is less,we have the therapeutic effect of curcumin in subarachnoid hem-orrhage to do a research,mainly from the anti-inflammatory and anti oxidative stress reaction,proof of curcumin in SAH,have the effect that reduce inflammation and oxidative stress reaction.Objective:1.Observe and record the change of neural function after acute subarachnoid hemo-rrhage,the research of curcumin by the effect of anti inflammatory response after SAH,inhibition of glutamate and inhibit the action of the nerve cell apoptosis.2.Study of curcumin in the role of oxidation stress reaction after SAH,mainly mani-fested in inhibiting oxidation product,and raise the content of the inhibitor.Methods:1.More see aneurysm rupture in clinical hemorrhagic subarachnoid hemorrhage,usi-ng intravascular puncture method in this experiment in the model to simulate the clini-cal pathological and physiological changes of acute hemorrhagic stroke.2.Choose adult male SD rats weight at about 300³20g,SAH model using intrav-ascular puncture the rule of law.By the random method the animals were divided into normal group,control group,SAH+curcumin treatment group,SAH+corn oil group.3.24 h after SAH,fast break in the brain,used to do:determination of the degree of brain edema;Detection of brain inflammation factors and chemokines changes such as:IL-1 beta,IL-6,the content of TNF alpha,etc;Product testing oxidative stress changes such as:ROS,MDA,3-NT,8-OHDG,etc;Detecting the change of the permeability of blo-od brain barrie,etc.4.SAH after 24 hours,with the heart inverse take cerebral perfusion method,make it frozen brain slices,used to do:detection of nerve cells apoptosis and changes in ED-1,etc.Determine the content of glutamate in 48 h to collect cerebrospinal fluid changes.Result:Curcumin treatment group can obviously improve neurologic deficits after SAH.Compared with control group,SAH brain tissue inflammatory cells infil-tration+corn oil group was obviously increased,the expression of inflammatory factor in brain tissue,brain water content increased,BBB permeability increase,the nerve cell apoptosis increased;The oxidation products of brain tissue increased,antioxidant content decreases.SAH+curcumin treatment group,the inflammatory cytokines can be seen infiltrating through the reduction,reduce brain edema degree,has improved the permeability of blood brain barrier,nerve cells apoptosis reduced;Oxidation products decreases,antioxidant content incre asi-ng.Conclusion:Curcumin can relieve after SAH cause inflammatory reaction and inhibit oxidative stress reaction,main performance in reducing brain water content,restrain and reduce the expression of inflammatory factors,improve the permeability of BBB,inhibit the apoptosis of nerve cells,the brain has certain protective effect after SAH.