Damage Of Radioative Particle ¹²⁵I Plane Model To Lung Cancer A549 Cells

Objectives:To investigate the damage effect of ¹²⁵I radioactive particles on A549 cells in isolated lung cancer,compared with different distances of ¹²⁵I radioactive particles to lung cancer A549 cell damage degree.The damage effect of ¹²⁵I radioactive particles of lung cancer in A549 cells was compared with different attenuation states.For clinical radioactive particles¹²⁵I brachytherapy to treat lung cancer with a more precise basis for radiation doses.Methods:Making radiation patches,made of 5 ¹²⁵I plaque design.The plaque itself consists of 5seeds,conrned within a 35mm assembly fabricated from cross-linked polystyrene,4 seeds are equally spaced within recesses around the circumference of a 30mm diameterdisc.The lung cancer A549 cells grown in good condition are evenly planted in the corresponding area of 96 orifice plate,placing the 96 orifice plate parallel to the radiation plane,the experimental group is divided into 1mm,5mm,10mm according to the distance,and the blank control group is set.Under the condition of proper culture,continuous brachytherapy48h,irradiation conditions continued to cultivate 24h,MTT colorimetric assay for cell survival.A549 cells grown in good condition of lung cancer were evenly planted in a35mm petri dish,placing the 35mm petri dish in parallel to the radiation plane,the experimental group is divided into 5mm,10mm according to the distance,and sets a blank control,suitable culture conditions under continuous near-distance irradiation 48h,the removal of irradiation conditions continue to cultivate 24h,flow cytometry detection cell apoptosis and apoptosis cycle.A549 cells of lung cancer with good growth status in a35mm petri dish,the culture dish is added to the sterile lid glass.To make the wall grow,the experimental group is divided into 1mm,5mm,10mm,blank control,suitable culture conditions for continuous irradiation of 48h days,the removal of irradiation conditions to cultivate 24h days,he staining and immunofluorescence staining,observation of cell morphology changes,and immune fluorescence situation.Results:Materials and methods:In vitro ¹²⁵I particle plane could damage the cells in the experimental group?P<0.05?was statistically significant;compared to the experimental group,¹²⁵I particle plane A549 cell apoptosis and the degree of particle distance on plane distance,1mm distance the most important group of apoptosis followed by 5mm distance group,10mm distance?P<0.05?group,with statistical significance.Flow cytometry was used to detect the cell cycle,and the effect of radioactive particles on A549 cells was significant in G2 and M phase?P<0.05?.Cell apoptosis was detected by flow cytometry,and the apoptosis of ¹²⁵I cells in vitro was significant in the early stage of apoptosis?P<0.05?.The comparison between the two groups showed that the cell damage was related to the particle attenuation,and the cell damage in the early stage?the same irradiation distance?was higher than that in the later stage,?P<0.05?.HE staining,the experimental group can see the obvious apoptotic cells and apoptotic bodies,the normal group no obvious apoptotic cells.Immunofluorescence staining showed that the cells in the experimental group were stained with green and red,while the control group did not see the green fluorescence and red staining.Conclusion:Iodine-125(¹²⁵I)radioactive particles can damage the lung cancer A549 cells.The closer the distance is,the more serious the damage is.The activity of ¹²⁵I radioactive particles?decay?has an effect on the damage of lung cancer A549 cells,the higher the early activity,the heavier the injury.¹²⁵I radioactive particles block A549 cells in G2 and M phase.¹²⁵I radioactive particles lead to the apoptosis of A549 cells,and the apoptotic bodies can be seen by HE staining.