| Background and ObjectivePreeclampsia(PE)is the severe phase of hypertension in pregnancy,which is difficult to reverse once it occurs.And it is one of the main causes of maternal and perinatal morbidity and mortality and seriously threats maternal and infant health.At present,there is no reliable biological index to predict the occurrence and development of PE.A? protein is a pathological hydrolyzed product of amyloid precursor protein.The deposition of A? protein is not only related to the degeneration of neurons,but also can cause micro-circulation changes.A few of domestic and foreign researches found that A? protein had been detected in the placenta and urine of PE pregnant women.On that case,a hypothesis had been putting forward that A?protein in urine of pregnant women may become a potential biological marker of predicting PE.We compared the difference of the A? protein content in urine of normal pregnant women and preeclampsia patients by a novel fluorescent molecular probe technology that specifically recognized A? protein to detect the content in urine of pregnant women.Then found the correlation between PE and the A? protein in the urine of pregnant women,and discussed whether or not A? protein could be used as a biological maker for predicting preeclampsia.It is of great significance to make early and simple screening of PE possible to reduce adverse outcomes of maternal and infant.Materials and Methods1.Research object:Urine samples were collected from thirty normal pregnant women and thirty patients with preeclampsia,who were both diagnosed and eventually terminated pregnancies in our hospital from October 2018 to March 2019.Then they were divided into normal pregnant women and preeclampsia.2.Specimen preparation:Maternal morning urine was collected and stored in a refrigerator at eighty degree below zero.A.Packaging:the samples were thawed in the incubator,sorted and packed with a two milliliter centrifuge tube,and then placed in the refrigerator at eighty degree below zero for standby application.B.Centrifuge:Put a tube of each sample in the heat preservation box slowly thawed,then were centrifuged at 5340 RPM under four degree for five minutes with high-speed refrigerated Centrifuge.Finally,the supernatant were reversed for later use.3.Probe synthesis:A novel probe was synthesized by modifying anthocyanin dye,which can specifically recognize the A? protein monomer and show increased fluorescence intensity after combined.Then the optimal probe concentration,the optimal absorption wavelength and the optimal excitation wavelength were selected.After the probe was added into the urine sample and mixed,the absorbance was measured with an enzyme marker.Finally according to the absorbance intensity,the content of A? protein of the two groups of samples was expressed.4.Statistical analysis:The differences in the level of A? protein concentration in urine between the normal pregnant women and the preeclampsia were compared,and then the significance of the differences in the level of A? protein in urine between the two group was analyzed by statistical method,and the P value was calculated.The difference was considered statistically when P value was less than 0.05 and statistically significant when P value less than 0.01.And it was considered to be discrepancy in A? protein content between the two group when P value was less than 0.05.ResultsCompared with the normal pregnancy group,the fluorescence intensity of molecular probe in the urine of pregnant women with preeclampsia group increased significantly.ConclusionThe comparison of the intensity of molecular fluorescence in the urine of the preeclampsia group and the normal group showed that the content of A? protein in the urine of the preeclampsia group was significantly higher than that of the normal pregnant group,indicating that A? protein had a certain role in the occurrence and development of preeclampsia disease and might become a biological marker for predicting the onset of preeclampsia. |
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