Establishment Of A Novel Hemorrhagic Shock Model In Rats And The Pathogenesis Of Shock Liver

Hemorrhagic shock(HS)is a common critical disease in surgery.At present,most of people's understanding of HS comes from animal experiments,so it is very important to establish a standardized animal model.According to the principle of hydraulic pressure,a new type of rat hemorrhagic shock model with automatic bleeding mode was constructed by using the blood storage device of syringe,and the mean arterial pressure(MAP)of each group was monitored,blood pressure and heart rate,blood loss,mortality,arterial blood gas analysis and pathological damage indexes of hippocampal neurons were used to evaluate the model.On the basis of the model,the necrosis of liver lobules was observed by HE staining,the changes of traditional biochemical indexes of liver function were detected,the content of energy metabolism index ATP and mitochondrial membrane potential(MMP)were detected objective:to explore the pathogenesis and potential mechanism of hepatic damage after hemorrhagic shock.The main results are as follows:1.The model of hemorrhagic shock in rats established by syringe blood bank can maintain stable hypotension for a long time and cause serious lactic acid poisoning.MAP could be accurately maintained at 31-35 mm Hg for at least 3 hours after shock;Lactate content increased significantly after shock and resuscitation(P<0.05);The maximum and final blood loss values were approximately the same,which could reach 60%of the total circulation blood volume and belonged to severe blood loss;2.The model of hemorrhagic shock in rats established by syringe blood bank can cause reliable tissue damage,and the model is treatable.After HE staining,pyknotic hippocampal neurons were observed in both shock and resuscitation groups;After 3 hours of shock,all rats in the non resuscitated group(RN group)died within 2 hours,and rats in the resuscitated group(RB group and RR group)survived within 3 hours;3.Shock liver occurs after the tissue is restored to perfusion.The results of HE staining showed that there was no necrosis in HS group;In the two groups treated with resuscitation,the hepatocytes were swollen,the sinuses were narrowed or disappeared,the cytoplasm was red and the cells were necrotic;The results of liver function test showed that there was no significant difference between HS group and Sham group(P>0.05);The level of ALT and AST in RB group and RR group increased significantly;The level of LDH in RR group was significantly increased(P<0.01),which was about 10 times of the normal value;The level of MDH in each group was very similar to that of LDH,and the level in RR group was about 10 times of the normal value;4.The occurrence of shock liver is accompanied by serious cellular energy metabolism disorder and mitochondrial damage.After 3 hours of shock,the content of ATP in hepatocytes decreased significantly(P<0.0001),although it increased significantly after resuscitation(P<0.05),it was still far lower than the normal level;Compared with Sham group,MMP decreased significantly after shock(P<0.05),in which the ratio of RB group decreased significantly(P<0.001);The ratio of red and green fluorescence decreased significantly after resuscitation(P<0.05);Compared with RB group,the ratio of RR group was slightly higher,but not statistically significant(P>0.05).