Research And Implementation Of Integrated Microfluidic System For Tuberculosis Detection

Tuberculosis is a chronic infectious disease caused by mycobacterium tuberculosis infection.People with active pulmonary tuberculosis are more infectious.Latent tuberculosis patients also have a certain probability of developing into active tuberculosis patients.Therefore,the detection and prevention of tuberculosis is of great significance.The traditional tuberculosis detection method,whether it is the scope of application of the test or the complexity of the operation,has significant room for improvement.In recent years,the emerging IGRA tuberculosis detection method can achieve highly sensitive detection of tuberculosis through its unique technical advantages and in vitro diagnostic methods,and significantly reduce the detection time,which is conducive to improving the level of tuberculosis detection and control.In order to achieve high-sensitivity detection in the IGRA tuberculosis detection method,the original sample needs to be cultivated for a long time,and then a series of operations such as pretreatment,dilution,reaction,and detection of the sample are performed to complete the detection process.Therefore,the steps of the entire detection process are cumbersome,the manual operation is complicated,and the efficiency of the detection process is low.As an integrated platform for biological reaction processes,the microfluidic chip has the characteristics of integration and miniaturization.In particular,with its internal fluid network structure,multi-step biochemical reaction processes can be integrated with each other,and through the pipeline reaction mode,the detection process can be automated or even intelligent.Therefore,combining microfluidic technology and IGRA tuberculosis detection technology,the integration and automation of IGRA tuberculosis detection through microfluidic system has an important practical role in improving the detection level of tuberculosis.This paper studies an integrated tuberculosis detection system based on microfluidic chips.First,based on the IGRA detection method,this paper researches and designs a multi-channel lateral flow strip fluorescence detection module to perform fluorescence detection on the reacted lateral flow strip;then researches and designs a microfluidic chip for tuberculosis detection,which integrates tuberculosis detection multiple reaction steps,including:whole blood culture,erythrocyte filtration,sample dilution,and chromatographic reaction,are integrated into the microfluidic chip to realize the automation of tuberculosis detection.Aiming at the microfluidic chip for tuberculosis detection,this paper researches and designs the supporting detection instruments and builds a complete integrated tuberculosis detection device.The research has realized the structure,software,hardware and photoelectric detection design of tuberculosis detection system,and constructed a portable detection device.The portable tuberculosis detection device can automatically complete the tuberculosis detection process according to the established process after adding the original test sample without manual operation,which significantly improves the overall level of IGRA tuberculosis detection.The actual sample test verifies the overall performance and technical feasibility of the integrated microfluidic system for tuberculosis detection,which lays a solid foundation for further improving the tuberculosis detection level.