Changes And Significance Of Serum Interleukin-33 Level In Newly Diagnosed Type 1 Diabetes Mellitus Children

Objective: To compare the serum IL-33 levels of newly diagnosed type 1 diabetes mellitus(T1DM)children with healthy controls,analyze the relationship between clinical data and IL-33 level in newly diagnosed type 1 diabetes children,and explore the role of IL-33 in the onset of T1 DM.Methods: To collect the clinical information and serum of 34 cases newly onset type 1 diabetes and 26 children for healthy check-up from Shengjing hospital affiliated to China medical university genetic metabolic pediatric endocrine ward.The IL-33 level of all serum were tested by ELISA.According to whether diabetic ketoacidosis happened,T1 DM autoantibodies and thyroid autoantibodies is positive,the newly diagnosed type 1 diabetes children are divided in to 2 groups respectively.Comparing IL-33 level in each group.To analyze whether the level of IL-33 was correlated with glucose metabolism,immunoglobulin and lymphocyte count in newly diagnosed T1 DM children.Results: The level of IL-33 in 34 patients[0.11(0.08～0.78)] was significantly lower than that in the healthy control group[0.91(0.14～2.20)],the difference was statistically significant(Z =-2.454,P<0.05).There was no statistically significant difference in IL-33 level between patients with or without diabetic ketoacidosis(Z =-0.227,P>0.05).There was no significant difference in the level of IL-33 in the presence or absence of autoantibody to T1DM(Z =-0.234,P>0.05).There was no significant difference in the production of thyroid autoantibodies or not(Z =-0.904,P>0.05).The level of IL-33 in patient was negatively correlated with IgA level(r=-0.406,P<0.05).Conclusion: Serum IL-33 level in newly diagnosed type 1 diabetes children was significantly lower than that in the healthy control group,and the low IL-33 level in newly diagnosis T1 DM children might raise the production of IgA by the way of humoral immunity to participate in the onset of T1 DM.