Expressions Of MMP2 And TIMP-4 In Colorectal Adenocarcinoma And Their Significance

Background: Colorectal adenocarcinoma(CRA)is a common malignant tumor of the digestive system.With the change of living habits in China,the incidence of colorectal adenocarcinoma(CRA)is on the rise,and most patients are in the middle and advanced stages when they are found.However,CRA is currently mainly used in surgery and supplemented with chemotherapy,and the treatment effect is not good.Therefore,early detection and early treatment of colorectal malignant tumors is an important way to prevent the progression of colorectal adenocarcinoma.The progress of malignant tumors is closely related to the degradation of tumor cells.Matrix metalloproteinase 2(MMP2)and tissue inhibitor of metalloproteinase-4(TIMP-4)are involved in the process of tumor cell interstitial degradation,during tumor cell migration,invasion,The progress of the transfer plays an important role.To clarify the role of MMP2 and TIMP-4 in the occurrence and development of colorectal adenocarcinoma and its mechanism can provide new research ideas for the early prevention and treatment of colorectal adenocarcinoma.Objective:To observe the expression of MMP2 and TIMP-4 proteins in colorectal adenocarcinoma,intraepithelial neoplasia and normal tissues,and to explore the role of their expressions in the occurrence and development of colorectal adenocarcinoma and their clinicopathological significance.Methods: 1.The m RNA expressions of MMP2 and Timp-4 in 20 cases of fresh colorectal adenocarcinoma after surgical resection and their paired normal intestinal mucosa were detected by Quantitative real-time PCR(q RT-PCR).;2.The protein expressions of MMP2 and Timp-4 in 20 cases of fresh colorectal adenocarcinoma after surgical resection and their paired normal intestinal mucosal tissues were detected by Western blot;3.MMP2 and TIMP-4 proteins were detected by immunohistochemistry in 90 cases of colorectal adenocarcinoma tissue,30 cases of high-grade intraepithelial neoplasia(HGIEN)tissue,30 cases of low-grade intraepithelial neoplasia(LGIN)tissue,and 50 cases corresponding to the expression of normal intestinal tissue;4.Analyze the relationship between MMP2 and TIMP-4 and the clinical pathological parameters of CRA,and explore the correlation between their expression.Results:1.The results of q RT-PCR indicated that there were statistically significant differences in the expression of MMP2 and Timp-4 m RNA in colorectal adenocarcinoma and the corresponding normal intestinal mucosa(P <0.05).2.Western blot experiments showed that MMP2 and TIMP-4 proteins were highly expressed in colorectal adenocarcinoma tissues and lowly expressed in normal intestinal mucosa tissues;3.The positive expression of MMP2 protein was localized in the cytoplasm of tumor cells,showing brownish yellow granules,and the positive cells were distributed in flakes.The positive expression rates of MMP2 protein in colorectal adenocarcinoma,HGIEN,LGIN,and normal intestinal tissue were 81.11%(73/90),63.33%(19/30),40.00%(12/30),and 18.00%(9 / 50)(P <0.001);TIMP-4protein-positive staining was localized in tumor cell cytoplasm,showing brownish-yellow granules,and positive cells were distributed in flakes.The positive rates in colorectal adenocarcinoma,HGIEN,LGIN,and normal intestinal mucosa were 87.78%(79/90),73.33%(22/30),40.00%(12/30),and 26.00% (13/50)(P <0.001);4.MMP2 protein expression was closely related to tumor size,lymph node metastasis,tumor differentiation,and TNM stage(P<0.05),and had no relationship with preoperative CEA,preoperative CA19-9,gender,age,and vascular infiltration(P ? 0.05);TIMP-4 protein expression is closely related to tumor size,tumor differentiation,and TNM stage(P < 0.05),and has nothing to do with gender,age,preoperative CEA,preoperative CA19-9,vascular invasion,and lymph node metastasis(P?0.05).5.There was a significant positive correlation between MMP2 and TIMP-4 protein expression in colorectal adenocarcinoma(r = 0.253,P = 0.016).Conclusion: MMP2 and TIMP-4 proteins are overexpressed in colorectal adenocarcinoma,and dysregulated MMP2 and TIMP-4 expression may participate in the occurrence and development of colorectal adenocarcinoma.