Biomarkers Of Type 2 Diabetes And Retinopathy Based On Metabonomics

Objective:Diabetic retinopathy is one of the common microangiopathy of diabetes,which is an important cause of blindness in adults,so early identification is of great significance to prevention and control.At present,there are no specific biomarkers of diabetic retinopathy,so this study is based on metabonomics to further search for biomarkers of type 2 diabetes mellitus and retinopathy.Method:The patients with type 2 diabetes diagnosed between July 2017 and June2018 were divided into simple diabetes group(DM group)and diabetic group with non-proliferative retinopathy group(DR group)according to the results of fundus photography,in the same period,the healthy physical examination patients were selected as the normal control group(NC group),69 cases per group.Based on ultra-high liquid chromatography-mass spectrometry system(UPLC-MS/MS),using a metabolomics approach to screen specific biomarkers.Result:Based on UPLC-MS/MS technology,532 small molecular metabolites were detected.Combined with orthogonal partial least squares discriminant analysis(OPLS-DA)and variable importance projection(VIP),12 differential metabolites were selected between DR group and DM group,34 differential metabolites between DM group and NC group,and 44 differential metabolites between DR group and NC group.Further analysis showed that 1-O-ferulaylquinic acid(1-O-FQA)was the most different in DM group,DR group and NC group(VIP values were 2.73 and 2.79,respectively),suggesting that it might be a potential biomarker.Conclusion:In this study,the UPLC-MS/MS technique combined with OPLS-DA was used to screen the differential metabolites between groups,and the potentialcharacteristic biomarkers of non-proliferative diabetic retinopathy(NPDR),1-O-FQA,were preliminarily screened,which provided valuable information for the early identification of NPDR.