| Objective:To observe the preventive effect of Bao Ru agent on ulcerative colitis-associated colon cancer(UCACC)model mice,to verify the preventive effect of Mongolian medicine Bao Ru agent on ulcerative colitis-associated colon cancer,for Mongolian medicine to prevent UCACC Provide reference experimental dataMethods:144 SPF grade BALB/c male mice were randomly divided into normal control group,Bao Ru agent low-dose group,Bao Ru agent middle-dose group,Bao Ru agent high-dose group,western medicine group(Mesalazine),model Group 6 groups Except for the normal control group,the remaining groups established a mouse model of colon cancer associated with ulcerative colitis through the combined application of azomethane(AOM)and three cycles of dextran sodium sulfate(DSS).From the 5th day of modeling,each treatment group was given a corresponding dose of drug enema to the end of the experiment,and the normal control group and model group were given equal doses of saline enema.AOM modeling started observe and record the general condition and body weight of mice every day.On the 28th and 70th days,the general condition,body weight,colon length,colon weight,unit intestine weight,general colon condition,colon pathological changes,tumor inhibition rate and tumor metastasis were observed in each groupResults:Mouse body weight:During the stage of tumor formation and tumor development,the body weight of the normal control group gradually increased,the body weight of the model group gradually decreased from the model building,and the body weight of the model group and the normal group began from the second week had a significant difference(P<0.05).From the third week,the body weight of the model group and each treatment group had a significant difference(P<0.05).There was a significant difference between the western medicine group and the other treatment groups at weeks 6 and 7(P<0.05)Colon length and weight:In the stage of tumor formation,compared with the normal control group,the length of the colon in the model group was significantly shortened and the weight was significantly increased.There was a significant difference between the two groups(P<0.05).Compared with the model group,the colon length and weight of the mice in each treatment group were significantly different(P<0.05)The colon length of the high-dose group,middle-dose group and western medicine groups did not have significant differences(P>0.05),Compared with the low-dose group,there was a significant difference(P<0.05)Unit intestinal weight:The stage of tumor formation and development stage,after modeling,the unit intestinal weight of mice was significantly higher than that of the normal control group.Compared with the normal control group,the intestinal weight of nodules in the model group increased significantly,and there was a significant difference between the two groups(P<0.05).The intestinal weight of mice in each treatment group was significantly different from that in the model group(P<0.05).At the stage of tumor formation,there was no significant difference in intestinal weight per unit of high-dose group,middle-dose group and western medicine group(P>0.05),compared with low-dose group(P<0.05).In the stage of tumor development,the low-dose group was significantly different from other treatment groups(P<0.05)General condition of the colon:In the stage of tumor formation and development,compared with the normal control group,the model group had a high score on the general condition of the colon,and there was a significant difference between the two groups(P<0.05).In the stage of tumor formation,there was no significant difference between the low-dose group and the model group(P>0.05).The other treatment groups were significantly different from the model group(P<0.05)Colonic pathological changes:In the tumor formation and development stages,compared with the normal control group,the model group had higher scores for colonic pathological changes,and there was a significant difference between the two groups(P<0.05).There were significant differences between the treatment groups and the model group(P<0.05).At the stage of tumor formation,there were significant differences between the Western medicine group,high-dose group and middle-dose group,low-dose group(P<0.05).At the stage of tumor development,there was a significant difference between the western medicine group,the high-dose group and the low-dose group(P<0.05)Tumor inhibition rate and tumor metastasis:In the tumor development stage,compared with the normal control group,the tumor colon of the model group was heavier,and there was a significant difference between the two groups(P<0.05)Western medicine group,high-dose,middle-dose and model group had significant difference(P<0.05),low-dose group and model group had no significant difference(P>0.05).Tumor metastasis,no tumor metastasis in each groupConclusion:1.Mongolian medicine Bao Ru agent has a certain relief effect on the weight loss,mucus bloody stool and other symptoms of UCACC model mice.2 Mongolian medicine Bao Ru agent has a certain repair effect on colon injury and colon pathological changes in UCACC model mice.3.Mongolian medicine Bao Ru agent can inhibit the formation and development of colon cancer in UCACC model mice.4.Mongolian medicine Bao Ru agent has a certain role in preventing and treating UCACC.Mongolian Medicine's prevention and treatment of UCACC has its own characteristics and advantages,and it is worthy of in-depth research and application. |
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