Study Of The All-glass PCR Chip System With A Micro Tube Reaction Chamber

With the deepening of globalization,the flows of people around the world have become more frequent and extensive,and the global spread of disease has posed a serious threat to mankind in the meanwhile.Thereby,online prediction,diagnosis,intervention and treatment of diseases in time are important for early detection of diseases and reduction of infections.To meet the needs of this application,Point-of-care testing(POCT)has been studied.Based on molecual biology technology,Polymerase chain reaction(PCR)can reliably achieve early detection of disease with highly accurate detection results.Currently,the widely used PCR instruments are very expensive with large sample size and high cost,which has hindered this technology from being applied in clinical and home.The PCR chip implemented by Micro-Electro-Mechanical System(MEMS)technology has the advantages of small size,low price,low sample consumption,fast temperature control,and short amplification reaction cycle,which can be used in gene analysis in POCT.The research goal of this paper is to produce a low-cost,high-performance all-glass tube structure PCR chip by the way of optimizing the structure of the PCR chip and simplifying the process flow.The peripheral embedded temperature control system has been integrated to achieve precise temperature control.Besides,the performance of the PCR chip has been verified by the DNA amplification reaction experiment.The research contents of this paper are as follows:1.Optimize the structure of existing PCR chips and streamline the processing technology of PCR chips.Including: replace the traditional temperature measuring electrode with PT100 temperature sensor to improve the accuracy and consistency of temperature measurement;make a reusable chip base with heating electrode and groove by micromachining(18mm × 12.5mm).2.Design the embedded temperature control system of PCR chip to complete the construction of PCR chip reaction platform.Design peripheral temperature detection circuit,power module circuit and drive circuit.Integrate PCR chip,embedded control system,LCD screen,the overall size of the system is 13 cm × 10.5cm.3.Implement the PCR chip temperature control algorithm to control the hardware circuit work,including UCOSIII operating system migration,real-time display of PCR chip temperature curve on LCD screen.Design temperature control finite state machine,multi-stage integral separation anti-saturation PID algorithm implementation and simulation.4.Verify the performance and work efficiency of the PCR chip through biological amplification experiments.The human oncogene was amplified and tested to obtain the heating rate of 6?C/s and the cooling rate of 3?C/s.The results of the electrophoresis tests on the amplified products show that compared with the conventional thermal cycler(ProFlex PCR System),the PCR chip of this study can obtain excellent amplification results.